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Team:Paris/July 14
From 2008.igem.org
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==Results of the transformation== | ==Results of the transformation== | ||
| - | * Positive control : | + | * Positive control : pUC19 (1µl; 10pg/ml) --> No transformation |
* Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice | * Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice | ||
* Biobricks 2008 : --> No transformation | * Biobricks 2008 : --> No transformation | ||
Conclusion : Cells are competents, because there is positive transformation | Conclusion : Cells are competents, because there is positive transformation | ||
Hypothesis : problem with the protocol of DNA extraction from the registery 2008 | Hypothesis : problem with the protocol of DNA extraction from the registery 2008 | ||
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==Culture of Cells== | ==Culture of Cells== | ||
| - | * Culture of DH5ɑ in 5ml of LB without Antibiotics | + | * Culture of DH5ɑ in 5ml of LB without Antibiotics, O/N at 37°C |
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==Preparation of medium and solutions== | ==Preparation of medium and solutions== | ||
Latest revision as of 10:08, 16 July 2008
Results of the transformation
Conclusion : Cells are competents, because there is positive transformation
Hypothesis : problem with the protocol of DNA extraction from the registery 2008
Culture of Cells
Preparation of medium and solutions
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