Team:Paris/July 21

From 2008.igem.org

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Latest revision as of 15:29, 21 July 2008

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Quantitative analysis of DNA

Electrophoresis

Gel : 0.8 % agarose
10 µL Quick-Load 1 kb DNA Ladder
2 µL Bromophenol Blue 5X + 2 µL DNA

Gel 1

Band	Biobricks	Clone	Expected size	Measured size
1	Ladder 1 kb
2	B0034 : Problem !!!	1	2.091 kb	~ 1.6 kb
3	B0034 : Problem !!!	2	2.091 kb	~ 1.6 kb
4	B0034 : Problem !!!	3	2.091 kb	~ 3 kb
5	R0040 : Problem !!!	1	2.133 kb	~ 1.7 kb
6	R0040 : Problem !!!	2	2.133 kb	~ 1.7 kb

Gel 2

Band	Biobricks	Clone	Expected size	Measured size
1	Ladder 1 kb
2	J23109 : Problem !!!	1	3.037 kb	~ 1.7 kb
3	J23109 : Problem !!!	2	3.037 kb	~ 5 kb
4	J23109 : Problem !!!	3	3.037 kb	~ 1.7 kb
5	C0179 : Problem !!!	1	2.802 kb	> 10 kb
6	C0179 : Problem !!!	2	2.802 kb	~ 5 kb
7	C0179 : Problem !!!	3	2.802 kb	~ 5 kb

@@ Line 3: / Line 3: @@
 ==Quantitative analysis of DNA==
 ===Electrophoresis===
+Gel : 0.8 % agarose<br>
+µL Quick-Load 1 kb DNA Ladder<br>
+µL Bromophenol Blue 5X + 2 µL DNA<br>
 ====Gel 1====
-[[Image:KR000016.jpg‎|right|250px]]
+[[Image:KR000016.jpg‎|right|200px]]
 {| border="1"
 |align="center"|'''Band'''
@@ Line 19: / Line 27: @@
 |-
 |align="left"|2
-|align="left"|E0040 : Problem !!!
+|align="left"|B0034 : Problem !!!
 |align="center"|1
-|align="center"|2.799 kb
+|align="center"|2.091 kb
-|align="center"|1.5 kb < size < 2.0 kb
+|align="center"|~ 1.6 kb
 |-
 |align="left"|3
-|align="left"|E0040 : Problem !!!
+|align="left"|B0034 : Problem !!!
 |align="center"|2
-|align="center"|2.799 kb
+|align="center"|2.091 kb
-|align="center"|1.5 kb < size < 2.0 kb
+|align="center"|~ 1.6 kb
 |-
 |align="left"|4
-|align="left"|J23101
+|align="left"|B0034 : Problem !!!
-|align="center"|1
+|align="center"|3
-|align="center"|3.037 kb
+|align="center"|2.091 kb
 |align="center"|~ 3 kb
 |-
 |align="left"|5
-|align="left"|J23101 : Problem !!!
+|align="left"|R0040 : Problem !!!
+|align="center"|1
+|align="center"|2.133 kb
+|align="center"|~ 1.7 kb
+|-
+|align="left"|6
+|align="left"|R0040 : Problem !!!
 |align="center"|2
+|align="center"|2.133 kb
+|align="center"|~ 1.7 kb
+|-
+|}
+====Gel 2====
+[[Image:KR000018.jpg‎|right|200px]]
+{| border="1"
+|align="center"|'''Band'''
+|align="center"|'''Biobricks'''
+|align="center"|'''Clone'''
+|align="center"|'''Expected size'''
+|align="center"|'''Measured size'''
+|-
+|align="left"|1
+|align="left"|Ladder 1 kb
+|align="center"|&nbsp;
+|align="center"|&nbsp;
+|align="center"|&nbsp;
+|-
+|align="left"|2
+|align="left"|J23109 : Problem !!!
+|align="center"|1
 |align="center"|3.037 kb
 |align="center"|~ 1.7 kb
 |-
-|align="left"|6
+|align="left"|3
-|align="left"|J23101 : Problem !!!
+|align="left"|J23109 : Problem !!!
+|align="center"|2
+|align="center"|3.037 kb
+|align="center"|~ 5 kb
+|-
+|align="left"|4
+|align="left"|J23109 : Problem !!!
 |align="center"|3
 |align="center"|3.037 kb
 |align="center"|~ 1.7 kb
+|-
+|align="left"|5
+|align="left"|C0179 : Problem !!!
+|align="center"|1
+|align="center"|2.802 kb
+|align="center"|> 10 kb
+|-
+|align="left"|6
+|align="left"|C0179 : Problem !!!
+|align="center"|2
+|align="center"|2.802 kb
+|align="center"|~ 5 kb
+|-
+|align="left"|7
+|align="left"|C0179 : Problem !!!
+|align="center"|3
+|align="center"|2.802 kb
+|align="center"|~ 5 kb
 |-
 |}
 ===Measurement of DNA concentration===

Team:Paris/July 21

From 2008.igem.org

Latest revision as of 15:29, 21 July 2008

Contents

Quantitative analysis of DNA

Electrophoresis

Gel 1

Gel 2

Measurement of DNA concentration