Team:Paris/July 21

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==Quantitative analysis of DNA==
==Quantitative analysis of DNA==
===Electrophoresis===
===Electrophoresis===
-
====Gel 1====
 
Gel : 0.8 % agarose<br>
Gel : 0.8 % agarose<br>
-
10µL Quick-Load 1kb DNA Ladder<br>
+
10 µL Quick-Load 1 kb DNA Ladder<br>
-
2µL + 2µL DNA<br>
+
2 µL Bromophenol Blue 5X + 2 µL DNA<br>
 +
 
 +
====Gel 1====
[[Image:KR000016.jpg‎|right|200px]]
[[Image:KR000016.jpg‎|right|200px]]
Line 58: Line 59:
====Gel 2====
====Gel 2====
-
Gel : 0.8 % agarose<br>
 
-
10µL Quick-Load 1kb DNA Ladder<br>
 
-
2µL + 2µL DNA<br>
 
[[Image:KR000018.jpg‎|right|200px]]
[[Image:KR000018.jpg‎|right|200px]]

Latest revision as of 15:29, 21 July 2008

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Contents

Quantitative analysis of DNA

Electrophoresis

Gel : 0.8 % agarose
10 µL Quick-Load 1 kb DNA Ladder
2 µL Bromophenol Blue 5X + 2 µL DNA

Gel 1

KR000016.jpg


Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 B0034 : Problem !!! 1 2.091 kb ~ 1.6 kb
3 B0034 : Problem !!! 2 2.091 kb ~ 1.6 kb
4 B0034 : Problem !!! 3 2.091 kb ~ 3 kb
5 R0040 : Problem !!! 1 2.133 kb ~ 1.7 kb
6 R0040 : Problem !!! 2 2.133 kb ~ 1.7 kb

Gel 2

KR000018.jpg
Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 J23109 : Problem !!! 1 3.037 kb ~ 1.7 kb
3 J23109 : Problem !!! 2 3.037 kb ~ 5 kb
4 J23109 : Problem !!! 3 3.037 kb ~ 1.7 kb
5 C0179 : Problem !!! 1 2.802 kb > 10 kb
6 C0179 : Problem !!! 2 2.802 kb ~ 5 kb
7 C0179 : Problem !!! 3 2.802 kb ~ 5 kb

Measurement of DNA concentration
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