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Revision as of 14:17, 28 July 2008 (view source) Fanny.c (Talk | contribs) (New page: === Results of extraction : Electrophoresis === conditions : * 10µl of ladder 1 kb * 2µl of extraction added with 2µl of loading Dye 6x * migration ~30min at 100W * Gel 1, 2, 3, 4...) ← Older edit		Latest revision as of 16:10, 28 July 2008 (view source) Fanny.c (Talk | contribs) (Replacing page with '{{Paris/Calendar_Links|July 26|July 28}}')
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-		+	{{Paris/Calendar_Links|July 26|July 28}}
-		+
-	=== Results of extraction : Electrophoresis ===	+
-		+
-	conditions :	+
-	* 10µl of ladder 1 kb	+
-	* 2µl of extraction added with 2µl of loading Dye 6x	+
-	* migration ~30min at 100W	+
-	* Gel 1, 2, 3, 4, 5 = '''0.8%'''	+
-	* Gel 6 = '''1,5%'''	+
-		+
-		+
-	gel1 [[Image:080728gel_1.jpg| gel1|200px]]	+
-	gel2 [[Image:080728gel_2.jpg| gel2|200px]]	+
-	gel3 [[Image:080728gel_3.jpg| gel3|200px]]	+
-	gel4 [[Image:080726gel_4.jpg| gel4|200px]]<br>	+
-		+
-		+
-	gel5 [[Image:‎080726gel_5.jpg| gel5|100px]]	+
-	gel6 [[Image:‎080726gel_6.jpg| gel6|100px]]	+
-		+
-		+
-		+
-	{| border="1"	+
-	|align=center|'''Name'''	+
-	|align=center|'''BioBrick'''	+
-	|align=center|'''Tube N°'''	+
-	|align=center|'''Enz 1'''	+
-	|align=center|'''Enz 2'''	+
-	|align=center|'''Obs'''	+
-	|align=center|'''Exp Size Matrix'''	+
-	|align=center|'''Exp Size BB'''	+
-	|align=center|'''Mea Size Matrix'''	+
-	|align=center|'''Mea Size BB'''	+
-	|align=center|'''Gel'''	+
-	|align=center|'''Band'''	+
-	|-	+
-	! rowspan="5" style="background: #ccccff;" | D100	+
-	! rowspan="5" style="background: #ccccff;" | B0034	+
-	|align=center|1	+
-	! rowspan="2"| XbaI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BI	+
-	! rowspan="2"| 2057 pb	+
-	! rowspan="2"| 34 pb	+
-	! rowspan="2"| 1800 pb	+
-	! rowspan="2"| 40 pb	+
-	! rowspan="1"| 6	+
-	! rowspan="1"| 2	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 6	+
-	! rowspan="1"| 3	+
-	|-	+
-	|align=center|3	+
-	|align=center| EcoRI	+
-	|align=center| XbaI	+
-	|align=center| FV	+
-	|align=center| 2076 pb	+
-	|align=center| 15 pb	+
-	|align=center| 2000 pb	+
-	|align=center| -	+
-	|align=center| 1	+
-	|align=center| 2	+
-	|-	+
-	|align=center|4	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2077 pb	+
-	! rowspan="2"| 14 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| -	+
-	|align=center| 1	+
-	|align=center| 3	+
-	|-	+
-	|align=center|5	+
-	|align=center| 1	+
-	|align=center| 4	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;" | D101	+
-	! rowspan="2" style="background: #ccccff;" | J23101	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2100 pb	+
-	! rowspan="2"| 883 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 700 pb	+
-	|align=center| 1	+
-	|align=center| 5	+
-	|-	+
-	|align=center|2	+
-	|align=center| 1	+
-	|align=center| 6	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;" | D102	+
-	! rowspan="2" style="background: #ccccff;" | J23109	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2100 pb	+
-	! rowspan="2"| 883 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 700 pb	+
-	! rowspan="1"| 1	+
-	! rowspan="1"| 7	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 1	+
-	! rowspan="1"| 8	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;" | D103	+
-	! rowspan="2" style="background: #ccccff;" | R0079	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2222 pb	+
-	! rowspan="2"| 14 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| -	+
-	|align=center| 1	+
-	|align=center| 9	+
-	|-	+
-	|align=center|2	+
-	|align=center| 1	+
-	|align=center| 10	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;" | D104	+
-	! rowspan="2" style="background: #ccccff;" | R0040	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2119 pb	+
-	! rowspan="2"| 14 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| -	+
-	|align=center| 1	+
-	|align=center| 11	+
-	|-	+
-	|align=center|2	+
-	|align=center| 1	+
-	|align=center| 12	+
-	|-	+
-	! rowspan="4" style="background: #ccccff;" | D105	+
-	! rowspan="4" style="background: #ccccff;" | S03154	+
-	|align=center|1	+
-	! rowspan="1"| SpeI	+
-	! rowspan="1"| PstI	+
-	! rowspan="1"| BV	+
-	! rowspan="1"| 2750 pb	+
-	! rowspan="1"| 14 pb	+
-	|align=center| 3000 pb	+
-	|align=center| -	+
-	|align=center| 2	+
-	|align=center| 5	+
-	|-	+
-	|align=center|2	+
-	! rowspan="2"| XbaI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BI	+
-	! rowspan="2"| 2057 pb	+
-	! rowspan="2"| 707 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 700 pb	+
-	|align=center| 2	+
-	|align=center| 2	+
-	|-	+
-	|align=center|3	+
-	|align=center| 2	+
-	|align=center| 3	+
-	|-	+
-	|align=center|4	+
-	| align=center|EcoRI	+
-	| align=center|SpeI	+
-	| align=center|FI	+
-	| align=center| 2056 pb	+
-	| align=center| 708 pb	+
-	|align=center| 2000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 2	+
-	|align=center| 4	+
-	|-	+
-	! rowspan="4" style="background: #ccccff;" | D106	+
-	! rowspan="4" style="background: #ccccff;" | S03879	+
-	|align=center|1	+
-	| align=center|SpeI	+
-	| align=center|PstI	+
-	| align=center|BV	+
-	| align=center| 2768 pb	+
-	| align=center|14 pb	+
-	|align=center| 2700 pb	+
-	|align=center| -	+
-	|align=center| 2	+
-	|align=center| 6	+
-	|-	+
-	|align=center|2	+
-	! rowspan="2"| XbaI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BI	+
-	! rowspan="2"| 2057 pb	+
-	! rowspan="2"| 725 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 700 pb	+
-	|align=center| 2	+
-	|align=center| 7	+
-	|-	+
-	|align=center|3	+
-	|align=center| 2	+
-	|align=center| 8	+
-	|-	+
-	|align=center|4	+
-	| align=center|EcoRI	+
-	| align=center|SpeI	+
-	| align=center|FI	+
-	| align=center| 2756 pb	+
-	| align=center| 726 pb	+
-	|align=center| 3000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 2	+
-	|align=center| 9	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;" |D107	+
-	! rowspan="2" style="background: #ccccff;" |C0079	+
-	|align=center| 1	+
-	|align=center|EcoRI	+
-	|align=center|SpeI	+
-	|align=center|FI	+
-	|align=center| 4402 pb	+
-	|align=center| 779 pb	+
-	|align=center| 5000 pb	+
-	|align=center| 800 pb	+
-	|align=center| 2	+
-	|align=center| 10	+
-	|-	+
-	|align=center| 2	+
-	|align=center|XbaI	+
-	|align=center|PstI	+
-	|align=center|BI	+
-	|align=center| 4003 pb	+
-	|align=center| 778 pb	+
-	|align=center| 5000 pb	+
-	|align=center| 800 pb	+
-	|align=center| 2	+
-	|align=center| 11	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D108	+
-	! rowspan="2" style="background: #ccccff;"|C0179	+
-	|align=center| 1	+
-	|align=center| EcoRI	+
-	|align=center| SpeI	+
-	|align=center| FI	+
-	|align=center| 4402 pb	+
-	|align=center| 746 pb	+
-	|align=center| 2000 pb	+
-	|align=center| 800 pb	+
-	|align=center| 2	+
-	|align=center| 12	+
-	|-	+
-	|align=center| 2	+
-	|align=center| XbaI	+
-	|align=center| PstI	+
-	|align=center| BI	+
-	|align=center| 4403 pb	+
-	|align=center| 745 pb	+
-	|align=center| 2000 pb	+
-	|align=center| 800 pb	+
-	|align=center| 3	+
-	|align=center| 2	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D109	+
-	! rowspan="2" style="background: #ccccff;"|E0030	+
-	|align=center| 1	+
-	|align=center|EcoRI	+
-	|align=center|SpeI	+
-	|align=center|FI	+
-	|align=center| 3166 pb	+
-	|align=center| 746 pb	+
-	|align=center| 3000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 3	+
-	|-	+
-	|align=center| 2	+
-	|align=center| XbaI	+
-	|align=center| PstI	+
-	|align=center| BI	+
-	|align=center| 3167 pb	+
-	|align=center| 745 pb	+
-	|align=center| 3000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 4	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D110	+
-	! rowspan="2" style="background: #ccccff;"|E0040	+
-	|align=center| 1	+
-	|align=center| EcoRI	+
-	|align=center| SpeI	+
-	|align=center| FI	+
-	|align=center| 2056 pb	+
-	|align=center| 743 pb	+
-	|align=center| 2000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 5	+
-	|-	+
-	|align=center| 2	+
-	|align=center| XbaI	+
-	|align=center| PstI	+
-	|align=center| BI	+
-	|align=center| 2057 pb	+
-	|align=center| 742 pb	+
-	|align=center| 2000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 6	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D111	+
-	! rowspan="2" style="background: #ccccff;"|E1010	+
-	|align=center| 1	+
-	|align=center| EcoRI	+
-	|align=center| SpeI	+
-	|align=center| FI	+
-	|align=center| 4402 pb	+
-	|align=center| 704 pb	+
-	|align=center| 5000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 7	+
-	|-	+
-	|align=center| 2	+
-	|align=center| XbaI	+
-	|align=center| PstI	+
-	|align=center| BI	+
-	|align=center| 4403 pb	+
-	|align=center| 703 pb	+
-	|align=center| 5000 pb	+
-	|align=center| 700 pb	+
-	|align=center| 3	+
-	|align=center| 8	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D116	+
-	! rowspan="2" style="background: #ccccff;"|J23100	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2100 pb	+
-	! rowspan="2"| 883 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 900 pb	+
-	! rowspan="1"| 3	+
-	! rowspan="1"| 9	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 3	+
-	! rowspan="1"| 10	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D117	+
-	! rowspan="2" style="background: #ccccff;"|J23107	+
-	|align=center|1	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2100 pb	+
-	! rowspan="2"| 883 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 900 pb	+
-	! rowspan="1"| 3	+
-	! rowspan="1"| 11	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 3	+
-	! rowspan="1"| 12	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D118	+
-	! rowspan="2" style="background: #ccccff;"|B0015	+
-	|align=center|1	+
-	! rowspan="2"|EcoRI	+
-	! rowspan="2"|XbaI	+
-	! rowspan="2"| FV	+
-	! rowspan="2"| 3303 pb	+
-	! rowspan="2"| 15 pb	+
-	! rowspan="2"| 3000 pb	+
-	! rowspan="2"| -	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 2	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 3	+
-	|-	+
-	! rowspan="2" style="background: #ccccff;"|D119	+
-	! rowspan="2" style="background: #ccccff;"|I0500	+
-	|align=center|1	+
-	! rowspan="2"|SpeI	+
-	! rowspan="2"|PstI	+
-	! rowspan="2"| FV	+
-	! rowspan="2"| 5621 pb	+
-	! rowspan="2"| 14 pb	+
-	! rowspan="2"| 6000 pb	+
-	! rowspan="2"| -	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 4	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 5	+
-	|-	+
-	! rowspan="5" style="background: #ccccff;" | D120	+
-	! rowspan="5" style="background: #ccccff;" | B0030	+
-	|align=center|1	+
-	! rowspan="2"| XbaI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BI	+
-	! rowspan="2"| 2057 pb	+
-	! rowspan="2"| 37 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 50 pb	+
-	! rowspan="1"| 6	+
-	! rowspan="1"| 4	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"| 6	+
-	! rowspan="1"| 5	+
-	|-	+
-	|align=center|3	+
-	! rowspan="1"|  EcoRI	+
-	! rowspan="1"|  XbaI	+
-	! rowspan="1"|  FV	+
-	! rowspan="1"| 2079 pb	+
-	! rowspan="1"|  15 pb	+
-	! rowspan="1"| 2200 pb	+
-	! rowspan="1"| -	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 6	+
-	|-	+
-	|align=center|4	+
-	! rowspan="2"| SpeI	+
-	! rowspan="2"| PstI	+
-	! rowspan="2"| BV	+
-	! rowspan="2"| 2080 pb	+
-	! rowspan="2"| 14 pb	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| -	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 7	+
-	|-	+
-	|align=center|5	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 8	+
-	|-	+
-	! rowspan="3" style="background: #ccccff;"|D121	+
-	! rowspan="3" style="background: #ccccff;"|E0422	+
-	|align=center|1	+
-	! rowspan="3"| XbaI	+
-	! rowspan="3"| PstI	+
-	! rowspan="3"| BI	+
-	! rowspan="3"| 2057 pb	+
-	! rowspan="3"| 939 pb	+
-	! rowspan="3"| 2000 pb	+
-	! rowspan="3"| 1100 pb	+
-	! rowspan="1"|  4	+
-	! rowspan="1"|  9	+
-	|-	+
-	|align=center|2	+
-	! rowspan="1"|  4	+
-	! rowspan="1"|  10	+
-	|-	+
-	|align=center|3	+
-	! rowspan="1"| 4	+
-	! rowspan="1"| 11	+
-	|-	+
-	! rowspan="3" style="background: #ccccff;"|D122	+
-	! rowspan="3" style="background: #ccccff;"|E0	+
-	|align=center|1	+
-	! rowspan="3"| XbaI	+
-	! rowspan="3"| PstI	+
-	! rowspan="3"| BI	+
-	! rowspan="3"| 2057 pb	+
-	! rowspan="3"| 900 pb	+
-	! rowspan="1"| 2000 pb	+
-	! rowspan="1"| 1000 pb	+
-	! rowspan="1"|  4	+
-	! rowspan="1"|  12	+
-	|-	+
-	|align=center|2	+
-	! rowspan="2"| 2000 pb	+
-	! rowspan="2"| 900 pb	+
-	! rowspan="1"|  5	+
-	! rowspan="1"|  2	+
-	|-	+
-	|align=center|3	+
-	! rowspan="1"| 5	+
-	! rowspan="1"| 3	+
-	|}	+
-		+
-		+
-	==> conclusion : all the digestion have succeed.....GREAT !	+

---

Latest revision as of 16:10, 28 July 2008

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