Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)
(→Results of the transformation)
(→Results of the transformation)
 
(One intermediate revision not shown)
Line 66: Line 66:
|-
|-
|align="center"|L123
|align="center"|L123
-
|align="center"|RBS lasI - ecfp<br>D107 (BV) - D131 (BI)
+
|align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI)
|align="center"|Amp
|align="center"|Amp
|align="center"|> 4000
|align="center"|> 4000
Line 80: Line 80:
|}
|}
-
 
+
[[Image:transfo.jpg|frame|center|L101]]
==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.
==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

Latest revision as of 17:09, 13 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →


Transformation of 1st August

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
puc19 positive control Amp - OK
L100 rbs TetR - ECFP
D110 (BV) - D130 (BI) 		Amp 608 No OK
L101 rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		Amp > 4000 GFP positive OK
L113 AracpBAD - ecfp
D126 (BV) - D130 (BI) 		Kana 9
L114 AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		Kana 9
L120 tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		Amp 74
L122 RBS-lasI - ecfp
D107 (BV) - D130 (BI) 		Amp 4 No OK
L123 RBS lasI - GFP tripart
D107 (BV) - D131 (BI) 		Amp > 4000 No OK
L126 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		Amp 944 No OK
L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

Retrieved from "http://2008.igem.org/Team:Paris/August_2"