Team:Paris/August 24
From 2008.igem.org
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='''Construction for synchronization'''= | ='''Construction for synchronization'''= | ||
+ | =='''Gel extraction'''== | ||
[[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]] | [[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]] | ||
+ | |||
+ | Gel 1 (no image sorry, we forgot to save it!) | ||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Well''' | ||
+ | |1 | ||
+ | |2 | ||
+ | |3 | ||
+ | |4 | ||
+ | |5 | ||
+ | |6 | ||
+ | |7 | ||
+ | |8 | ||
+ | |9 | ||
+ | |10 | ||
+ | |11 | ||
+ | |12 | ||
+ | |- | ||
+ | |'''Sample''' | ||
+ | |1kb ladder | ||
+ | |MP105.1 | ||
+ | |no sample | ||
+ | |D107 | ||
+ | |no sample | ||
+ | |D109 | ||
+ | |no sample | ||
+ | |MP106 | ||
+ | |no sample | ||
+ | |D110 | ||
+ | |no sample | ||
+ | |100pb ladder | ||
+ | |- | ||
+ | |'''Expected size (pb)''' | ||
+ | | - | ||
+ | |2764 | ||
+ | | - | ||
+ | |2750<br>14 | ||
+ | | - | ||
+ | |2056<br>708 | ||
+ | | - | ||
+ | |2782 | ||
+ | | - | ||
+ | |2768<br>14 | ||
+ | | - | ||
+ | | - | ||
+ | |- | ||
+ | |'''Measured size (pb)''' | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2800 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2750 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2000<br>700 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2800 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2750 | ||
+ | | - | ||
+ | | - | ||
+ | |} | ||
+ | |||
+ | Gel 2 | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Well''' | ||
+ | |1 | ||
+ | |2 | ||
+ | |3 | ||
+ | |4 | ||
+ | |5 | ||
+ | |6 | ||
+ | |7 | ||
+ | |8 | ||
+ | |9 | ||
+ | |10 | ||
+ | |11 | ||
+ | |12 | ||
+ | |- | ||
+ | |'''Sample''' | ||
+ | |no sample | ||
+ | |1kb ladder | ||
+ | |MP118.1 | ||
+ | |no sample | ||
+ | |D125 | ||
+ | |no sample | ||
+ | |MP143 | ||
+ | |no sample | ||
+ | |D163 | ||
+ | |no sample | ||
+ | |100pb ladder | ||
+ | |no sample | ||
+ | |- | ||
+ | |'''Expected size (pb)''' | ||
+ | | - | ||
+ | | - | ||
+ | |3318 | ||
+ | | - | ||
+ | |3303<br>15 | ||
+ | | - | ||
+ | |3322 | ||
+ | | - | ||
+ | |2432<br>890 | ||
+ | | - | ||
+ | | - | ||
+ | | - | ||
+ | |- | ||
+ | |'''Measured size (pb)''' | ||
+ | | - | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2900 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 3300 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2900 | ||
+ | | - | ||
+ | |style="background: #cbff7B"| 2500<br>900 | ||
+ | | - | ||
+ | | - | ||
+ | | - | ||
+ | |} | ||
+ | |||
+ | =='''Ligation'''== | ||
+ | [[Team:Paris/Notebook/Protocols#Ligation |Protocol]] | ||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Ligation Name''' | ||
+ | |'''Vector Name''' | ||
+ | |'''Volume Vector (µL)''' | ||
+ | |'''Insert''' | ||
+ | |'''Volume Insert (µL)''' | ||
+ | |- | ||
+ | |L159 | ||
+ | |D125 | ||
+ | |6 | ||
+ | |D109 | ||
+ | |3.86 | ||
+ | |- | ||
+ | |L162 | ||
+ | |D107 | ||
+ | |5 | ||
+ | |D163 | ||
+ | |5.3 | ||
+ | |- | ||
+ | |L163 | ||
+ | |D110 | ||
+ | |7.5 | ||
+ | |D163 | ||
+ | |5.32 | ||
+ | |- | ||
+ | |Control L159 | ||
+ | |D125 | ||
+ | |6 | ||
+ | | - | ||
+ | | - | ||
+ | |- | ||
+ | |Control L162 | ||
+ | |D107 | ||
+ | |5 | ||
+ | | - | ||
+ | | - | ||
+ | |- | ||
+ | |Control L163 | ||
+ | |D110 | ||
+ | |7.5 | ||
+ | | - | ||
+ | | - | ||
+ | |} |
Latest revision as of 12:58, 25 August 2008
Construction for synchronizationGel extractionGel 1 (no image sorry, we forgot to save it!)
Gel 2
Ligation
|