Team:Paris/August 24

From 2008.igem.org

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[[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]]
[[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]]
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Gel 1 (ni image sorry, we forgot to save it!)
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Gel 1 (no image sorry, we forgot to save it!)
{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
|'''Well'''
|'''Well'''
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| -  
| -  
|style="background: #cbff7B"| 2750
|style="background: #cbff7B"| 2750
 +
| -
 +
| -
 +
|}
 +
 +
Gel 2
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 +
{|border="1" style="text-align: center"
 +
|'''Well'''
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|1
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|2
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|3
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|4
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|5
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|6
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|7
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|10
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|11
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|12
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|-
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|'''Sample'''
 +
|no sample
 +
|1kb ladder
 +
|MP118.1
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|no sample
 +
|D125
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|no sample
 +
|MP143
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|no sample
 +
|D163
 +
|no sample
 +
|100pb ladder
 +
|no sample
 +
|-
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|'''Expected size (pb)'''
 +
| -
 +
| -
 +
|3318
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| -
 +
|3303<br>15
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| -
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|3322
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| -
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|2432<br>890
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| -
 +
| -
 +
| -
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|-
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|'''Measured size (pb)'''
 +
| -
 +
| -
 +
|style="background: #cbff7B"| 2900
 +
| -
 +
|style="background: #cbff7B"| 3300
 +
| -
 +
|style="background: #cbff7B"| 2900
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| -
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|style="background: #cbff7B"| 2500<br>900
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| -
 +
| -
 +
| -
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|}
 +
 +
=='''Ligation'''==
 +
[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
 +
 +
{|border="1" style="text-align: center"
 +
|'''Ligation Name'''
 +
|'''Vector Name'''
 +
|'''Volume Vector (µL)'''
 +
|'''Insert'''
 +
|'''Volume Insert (µL)'''
 +
|-
 +
|L159
 +
|D125
 +
|6
 +
|D109
 +
|3.86
 +
|-
 +
|L162
 +
|D107
 +
|5
 +
|D163
 +
|5.3
 +
|-
 +
|L163
 +
|D110
 +
|7.5
 +
|D163
 +
|5.32
 +
|-
 +
|Control L159
 +
|D125
 +
|6
 +
| -
 +
| -
 +
|-
 +
|Control L162
 +
|D107
 +
|5
 +
| -
 +
| -
 +
|-
 +
|Control L163
 +
|D110
 +
|7.5
| -
| -
| -
| -
|}
|}

Latest revision as of 12:58, 25 August 2008

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Construction for synchronization

Gel extraction

Protocol

Gel 1 (no image sorry, we forgot to save it!)

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample 1kb ladder MP105.1 no sample D107 no sample D109 no sample MP106 no sample D110 no sample 100pb ladder
Expected size (pb) - 2764 - 2750
14 		- 2056
708 		- 2782 - 2768
14 		- -
Measured size (pb) - 2800 - 2750 - 2000
700 		- 2800 - 2750 - -

Gel 2

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample no sample 1kb ladder MP118.1 no sample D125 no sample MP143 no sample D163 no sample 100pb ladder no sample
Expected size (pb) - - 3318 - 3303
15 		- 3322 - 2432
890 		- - -
Measured size (pb) - - 2900 - 3300 - 2900 - 2500
900 		- - -

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L159 D125 6 D109 3.86
L162 D107 5 D163 5.3
L163 D110 7.5 D163 5.32
Control L159 D125 6 - -
Control L162 D107 5 - -
Control L163 D110 7.5 - -
Retrieved from "http://2008.igem.org/Team:Paris/August_24"