Team:Paris/September 9

From 2008.igem.org

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{{Paris/Calendar_Links|September 8|September 10}}
{{Paris/Calendar_Links|September 8|September 10}}
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=Checking our ligases (following)=
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==Ligation (2 hours at 16°C)==
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*4 µL DNA
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*2 µL 10X buffer 2
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*1 µL T4 DNA ligase
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*13 µL water
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==Transformation==
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*Strain used: Chemically competent E. coli DH5 alpha cells
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*100 µL of cells
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*5 µL of ligation products
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*30 min on ice
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*heat shock: 45 s at 37°C
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*2 min on ice
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*900 µL of SOC
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*1 hour at 37°C
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*plating (1 µL or 100 µL) on LB plates containing ampicilline

Latest revision as of 14:26, 12 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Checking our ligases (following)

Ligation (2 hours at 16°C)

  • 4 µL DNA
  • 2 µL 10X buffer 2
  • 1 µL T4 DNA ligase
  • 13 µL water

Transformation

  • Strain used: Chemically competent E. coli DH5 alpha cells
  • 100 µL of cells
  • 5 µL of ligation products
  • 30 min on ice
  • heat shock: 45 s at 37°C
  • 2 min on ice
  • 900 µL of SOC
  • 1 hour at 37°C
  • plating (1 µL or 100 µL) on LB plates containing ampicilline
Retrieved from "http://2008.igem.org/Team:Paris/September_9"