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Team:Paris/August 28
From 2008.igem.org
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==Digestion== | ==Digestion== | ||
| - | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR- | + | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] |
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*pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | ||
| - | + | =Freezer empty= | |
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[[Team:Paris/Notebook/Protocols#Digestion |Protocol]] | [[Team:Paris/Notebook/Protocols#Digestion |Protocol]] | ||
We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and [[Team:Paris/Notebook/Freezer#Digestion Products |D125]]. | We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and [[Team:Paris/Notebook/Freezer#Digestion Products |D125]]. | ||
Latest revision as of 19:09, 9 September 2008
Transformation of OmpR*Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
Freezer empty |
