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Team:Paris/September 1
From 2008.igem.org
(Difference between revisions)
(→Construction of pFlhB - mRFP Tripart (LVA+)) |
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| - | =Construction of | + | =Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)= |
Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
| - | <br> | + | <br>==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted |
==Digestion== | ==Digestion== | ||
| Line 45: | Line 45: | ||
|16 | |16 | ||
|1.31 | |1.31 | ||
| + | |- | ||
| + | |D186 | ||
| + | |24 | ||
| + | |1.25 | ||
|} | |} | ||
| Line 59: | Line 63: | ||
|L168 | |L168 | ||
|D187 | |D187 | ||
| - | | | + | |2.5 |
|D186 | |D186 | ||
| - | | | + | |1 |
|- | |- | ||
|Control L168 | |Control L168 | ||
|D166 | |D166 | ||
| - | | | + | |2.5 |
| - | | - | ||
|} | |} | ||
Latest revision as of 14:49, 13 September 2008
Cloning of EnvZ*Transformation results of L165 (EnvZ*-pSB1A2) from August 29th
Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
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