Team:Paris/September 10

From 2008.igem.org

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(→Checking our ligases (following))
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=Checking our ligases (following)=
=Checking our ligases (following)=
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==Analysis of the ligation transformants==
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*Several colonies for the pUC19 transformation control
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*No colonies for the ligations
==Analysis of the ligation products by electrophoresis==
==Analysis of the ligation products by electrophoresis==
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|15
|15
|-
|-
-
|'''
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|'''sample'''
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|rowspan="5"|1 kb <br> DNA ladder
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|rowspan="5"|undigested DNA <br> MP101.1
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|colspan="12"|'''ligation products'''
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|rowspan="5"|100 bp <br> DNA ladder
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|-
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|'''temperature of ligation'''
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|colspan="8"|16°C
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|colspan="4"|4°C
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|-
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|'''ligase n°'''
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|colspan="4"|L1
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|colspan="4"|L2
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|colspan="2"|L1
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|colspan="2"|L2
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|-
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|'''duration of ligation'''
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|colspan="2"|2 h
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|colspan="2"|O/N
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|colspan="2"|2 h
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|colspan="6"|O/N
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|-
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|'''amount of ligase (units)'''
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|400 U
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|2 U
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|400 U
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|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|-
 +
|}

Latest revision as of 14:26, 12 September 2008

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Checking our ligases (following)

Analysis of the ligation transformants

  • Several colonies for the pUC19 transformation control
  • No colonies for the ligations

Analysis of the ligation products by electrophoresis

KR000310.JPG
well n° 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
sample 1 kb
DNA ladder 		undigested DNA
MP101.1 		ligation products 100 bp
DNA ladder
temperature of ligation 16°C 4°C
ligase n° L1 L2 L1 L2
duration of ligation 2 h O/N 2 h O/N
amount of ligase (units) 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U
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