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TUDelft/6 October 2008
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(New page: {{Template:TUDelftiGEM2008}} {{Template:TUDelftiGEM2008_menu_home}} {{Template:TUDelftiGEM2008_calendar}} =October 6th= A new colony PCR was done on the transformed cells. The gel is depi...) |
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| + | ==Colony PCR== | ||
A new colony PCR was done on the transformed cells. The gel is depicted in figure 1. AtoB, idi, ispA and BB_K115033 are listed in the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=TUDelft TUDelft registry]. Sizes of the parts + biobrick primer DNA should be: | A new colony PCR was done on the transformed cells. The gel is depicted in figure 1. AtoB, idi, ispA and BB_K115033 are listed in the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=TUDelft TUDelft registry]. Sizes of the parts + biobrick primer DNA should be: | ||
*atoB: 1504 | *atoB: 1504 | ||
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*K115033: 1604 | *K115033: 1604 | ||
| - | [[Image:TUDelftcolPCR061008.jpg|thumb|center|Figure | + | [[Image:TUDelftcolPCR061008.jpg|thumb|center|Figure 1. Colony PCR of atoB, idi, ispA and K115033. ~33 = K115033]] |
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| + | ==Growing thermoparts== | ||
| + | For miniprepping we will grow the thermoparts K115030, K115033 and K115035 ([https://2008.igem.org/Image:TUDelft0210083335.jpg first lane]) on Tetracycline. We decided not to grow a new K115032 as it didn't look any better as the one we're working with already. | ||
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{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Latest revision as of 10:24, 9 October 2008
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| [http://2008.igem.org/TUDelft/8_September_2008 8] | [http://2008.igem.org/TUDelft/9_September_2008 9] | [http://2008.igem.org/TUDelft/10_September_2008 10] | [http://2008.igem.org/TUDelft/11_September_2008 11] | [http://2008.igem.org/TUDelft/12_September_2008 12] | [http://2008.igem.org/wiki/index.php?title=TUDelft/13_September_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=TUDelft/14_September_2008&action=edit 14] |
| [http://2008.igem.org/TUDelft/15_September_2008 15] | [http://2008.igem.org/TUDelft/16_September_2008 16] | [http://2008.igem.org/TUDelft/17_September_2008 17] | [http://2008.igem.org/TUDelft/18_September_2008 18] | [http://2008.igem.org/TUDelft/19_September_2008 19] | [http://2008.igem.org/wiki/index.php?title=TUDelft/20_September_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=TUDelft/21_September_2008&action=edit 21] |
| [http://2008.igem.org/TUDelft/22_September_2008 22] | [http://2008.igem.org/TUDelft/23_September_2008 23] | [http://2008.igem.org/TUDelft/24_September_2008 24] | [http://2008.igem.org/TUDelft/25_September_2008 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_September_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=TUDelft/27_September_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_September_2008&action=edit 28] |
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| October | ||||||
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| [http://2008.igem.org/TUDelft/6_October_2008 6] | [http://2008.igem.org/TUDelft/7_October_2008 7] | [http://2008.igem.org/TUDelft/8_October_2008 8] | [http://2008.igem.org/TUDelft/9_October_2008 9] | [http://2008.igem.org/TUDelft/10_October_2008 10] | [http://2008.igem.org/wiki/index.php?title=TUDelft/11_October_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=TUDelft/12_October_2008&action=edit 12] |
| [http://2008.igem.org/TUDelft/13_October_2008 13] | [http://2008.igem.org/TUDelft/14_October_2008 14] | [http://2008.igem.org/TUDelft/15_October_2008 15] | [http://2008.igem.org/TUDelft/16_October_2008 16] | [http://2008.igem.org/TUDelft/17_October_2008 17] | [http://2008.igem.org/wiki/index.php?title=TUDelft/18_October_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=TUDelft/19_October_2008&action=edit 19] |
| [http://2008.igem.org/TUDelft/20_October_2008 20] | [http://2008.igem.org/TUDelft/21_October_2008 21] | [http://2008.igem.org/TUDelft/22_October_2008 22] | [http://2008.igem.org/TUDelft/23_October_2008 23] | [http://2008.igem.org/TUDelft/24_October_2008 24] | [http://2008.igem.org/wiki/index.php?title=TUDelft/25_October_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_October_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=TUDelft/27_October_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_October_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=TUDelft/29_October_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=TUDelft/30_October_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=TUDelft/31_October_2008&action=edit 31] | ||
October 6th
Colony PCR
A new colony PCR was done on the transformed cells. The gel is depicted in figure 1. AtoB, idi, ispA and BB_K115033 are listed in the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=TUDelft TUDelft registry]. Sizes of the parts + biobrick primer DNA should be:
- atoB: 1504
- idi: 868
- ispA: 1219
- K115033: 1604
Growing thermoparts
For miniprepping we will grow the thermoparts K115030, K115033 and K115035 (first lane) on Tetracycline. We decided not to grow a new K115032 as it didn't look any better as the one we're working with already.
