Team:Paris/July 17

From 2008.igem.org

(Difference between revisions)
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===Cloning of the transformations===
+
==Cloning of the transformations==
* Test 8 clones by Biobricks
* Test 8 clones by Biobricks
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===Screening PCR of the transformations===
+
==Screening PCR of the transformations==
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1µl Oligo R
1µl Oligo R
23µl H2O
23µl H2O
 +
 +
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===Protocol==
* 50µl of Mix PCR by tubes
* 50µl of Mix PCR by tubes
* 1 clone
* 1 clone
* Programme : 105°C -  for 28 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'
* Programme : 105°C -  for 28 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'

Revision as of 19:00, 17 July 2008

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Contents

Results of the transformations

Legende :

  • few , medium, a lot --> description of the number of CFU(Colony forming unit) produce by the transformation
  • A: Ampicilin, K: Kanmycin


Ref. Book Biobricks 2007 Antibio Results
- positive control A 2.400 CFU
- negative control A 0 CFU
2E_SP 1000 B0034 A few
10F_SP 1001 R0079 A medium
4F_SP 1013 C0079 K few
6H_SP 1001 C0179 A medium
6B_SP 1001 S03154 A 0 CFU
10F_SP 1002 J23101 A a lot
11F_SP 1002 J23109 A a lot
4C_SP 1000 R0040 A 2 CFU
2G_SP 1000 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot


Cloning of the transformations

  • Test 8 clones by Biobricks
  • Incubate O/N at 37°C


Screening PCR of the transformations

Mix for PCR (x76 tubes)

25µl Quick load 2x 1µl Oligo F 1µl Oligo R 23µl H2O


=Protocol

  • 50µl of Mix PCR by tubes
  • 1 clone
  • Programme : 105°C - for 28 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'
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