Team:Paris/July 21

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===Measurement of DNA concentration===
===Measurement of DNA concentration===

Revision as of 15:21, 21 July 2008

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Contents

Quantitative analysis of DNA

Electrophoresis

Gel 1

Gel : 0.8 % agarose
10µL Quick-Load 1kb DNA Ladder
2µL + 2µL DNA

KR000016.jpg


Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 B0034 : Problem !!! 1 2.091 kb ~ 1.6 kb
3 B0034 : Problem !!! 2 2.091 kb ~ 1.6 kb
4 B0034 : Problem !!! 3 2.091 kb ~ 3 kb
5 R0040 : Problem !!! 1 2.133 kb ~ 1.7 kb
6 R0040 : Problem !!! 2 2.133 kb ~ 1.7 kb

Gel 2

Gel : 0.8 % agarose
10µL Quick-Load 1kb DNA Ladder
2µL + 2µL DNA

KR000018.jpg
Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 J23109 : Problem !!! 1 3.037 kb ~ 1.7 kb
3 J23109 : Problem !!! 2 3.037 kb ~ 5 kb
4 J23109 : Problem !!! 3 3.037 kb ~ 1.7 kb
5 C0179 : Problem !!! 1 2.802 kb > 10 kb
6 C0179 : Problem !!! 2 2.802 kb ~ 5 kb
7 C0179 : Problem !!! 3 2.802 kb ~ 5 kb


Measurement of DNA concentration
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