From 2008.igem.org
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| | ===Electrophoresis=== | | ===Electrophoresis=== |
| | Gel : 0.8 % agarose<br> | | Gel : 0.8 % agarose<br> |
| - | 10µL Quick-Load 1kb DNA Ladder<br>
| + | 10 µL Quick-Load 1 kb DNA Ladder<br> |
| - | 2µL Bromophenol Blue 5X + 2µL DNA<br>
| + | 2 µL Bromophenol Blue 5X + 2 µL DNA<br> |
| | | | |
| | ====Gel 1==== | | ====Gel 1==== |
Revision as of 15:23, 21 July 2008
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Quantitative analysis of DNA
Electrophoresis
Gel : 0.8 % agarose
10 µL Quick-Load 1 kb DNA Ladder
2 µL Bromophenol Blue 5X + 2 µL DNA
Gel 1
| Band
| Biobricks
| Clone
| Expected size
| Measured size
|
| 1
| Ladder 1 kb
|
|
|
|
| 2
| B0034 : Problem !!!
| 1
| 2.091 kb
| ~ 1.6 kb
|
| 3
| B0034 : Problem !!!
| 2
| 2.091 kb
| ~ 1.6 kb
|
| 4
| B0034 : Problem !!!
| 3
| 2.091 kb
| ~ 3 kb
|
| 5
| R0040 : Problem !!!
| 1
| 2.133 kb
| ~ 1.7 kb
|
| 6
| R0040 : Problem !!!
| 2
| 2.133 kb
| ~ 1.7 kb
|
Gel 2
Gel : 0.8 % agarose
10µL Quick-Load 1kb DNA Ladder
2µL + 2µL DNA
| Band
| Biobricks
| Clone
| Expected size
| Measured size
|
| 1
| Ladder 1 kb
|
|
|
|
| 2
| J23109 : Problem !!!
| 1
| 3.037 kb
| ~ 1.7 kb
|
| 3
| J23109 : Problem !!!
| 2
| 3.037 kb
| ~ 5 kb
|
| 4
| J23109 : Problem !!!
| 3
| 3.037 kb
| ~ 1.7 kb
|
| 5
| C0179 : Problem !!!
| 1
| 2.802 kb
| > 10 kb
|
| 6
| C0179 : Problem !!!
| 2
| 2.802 kb
| ~ 5 kb
|
| 7
| C0179 : Problem !!!
| 3
| 2.802 kb
| ~ 5 kb
|
Measurement of DNA concentration
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