Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)

Revision as of 13:06, 24 July 2008

MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.

Name	Biobrick	Description
MP116 (=MP1)	J23100
MP117 (=MP2)	J23107
MP118 (=MP3)	B0015
MP119 (=MP4)	I0500
MP120 (=MP5)	B0030
MP121 (=MP6)	E0422
MP122 (=MP8)	E0840

Digestion

Name	Miniprep	Biobrick	Enzyme 1	Enzyme 2	Nber tube
D100	MP100	B0034	XbaI	PstI	2
			EcoRI	XbaI	1
			SpeI	PstI	2
D101	MP101	J23101	SpeI	PstI	2
D102	MP102	J23109	SpeI	PstI	2
D103	MP103	R0079	SpeI	PstI	2
D104	MP104	R0040	SpeI	PstI	2
D105	MP105	S03154	SpeI	PstI	1
			XbaI	PstI	2
			EcoRI	SpeI	1
D106	MP106	J23101	SpeI	PstI	1
			XbaI	PstI	2
			EcoRI	SpeI	1
D107	MP107	C0079	EcoRI	SpeI	1
D108	MP108	C0179	EcoRI	SpeI	1
D109	MP109	E0030	EcoRI	SpeI	1
D110	MP110	E0040	EcoRI	SpeI	1
D111	MP111	E1010	EcoRI	SpeI	1
D116	MP116(=MP1)	J23100	SpeI	PstI	2
D117	MP117(=MP2)	J23107	SpeI	PstI	2
D118	MP118(=MP3)	B0015	EcoRI	XbaI	2
D119	MP119(=MP4)	I0500	SpeI	PstI	2
D120	MP120(=MP5)	B0030	SpeI	PstI	2
			EcoRI	XbaI	1
			XbaI	PstI	2
D121	MP121(=MP6)	E0422	XbaI	PstI	3
D122	MP122(=MP8)	E0840	XbaI	PstI	3

@@ Line 152: / Line 152: @@
 |align="center"|1
 |-
-|align="center"|D107
+|align="center"|D108
-|align="center"|MP107
+|align="center"|MP108
-|align="center"|C0079
+|align="center"|C0179
 |align="center"|EcoRI
 |align="center"|SpeI
 |align="center"|1
 |-
+|align="center"|D109
+|align="center"|MP109
+|align="center"|E0030
+|align="center"|EcoRI
+|align="center"|SpeI
+|align="center"|1
+|-
+|align="center"|D110
+|align="center"|MP110
+|align="center"|E0040
+|align="center"|EcoRI
+|align="center"|SpeI
+|align="center"|1
+|-
+|align="center"|D111
+|align="center"|MP111
+|align="center"|E1010
+|align="center"|EcoRI
+|align="center"|SpeI
+|align="center"|1
+|-
+|align="center"|D116
+|align="center"|MP116(=MP1)
+|align="center"|J23100
+|align="center"|SpeI
+|align="center"|PstI
+|align="center"|2
+|-
+|align="center"|D117
+|align="center"|MP117(=MP2)
+|align="center"|J23107
+|align="center"|SpeI
+|align="center"|PstI
+|align="center"|2
+|-
+|align="center"|D118
+|align="center"|MP118(=MP3)
+|align="center"|B0015
+|align="center"|EcoRI
+|align="center"|XbaI
+|align="center"|2
+|-
+|align="center"|D119
+|align="center"|MP119(=MP4)
+|align="center"|I0500
+|align="center"|SpeI
+|align="center"|PstI
+|align="center"|2
+|-
+|align="center"|D120
+|align="center"|MP120(=MP5)
+|align="center"|B0030
+|align="center"|SpeI
+|align="center"|PstI
+|align="center"|2
+|-
+|align="center"|
+|align="center"|
+|align="center"|
+|align="center"|EcoRI
+|align="center"|XbaI
+|align="center"|1
+|-
+|align="center"|
+|align="center"|
+|align="center"|
+|align="center"|XbaI
+|align="center"|PstI
+|align="center"|2
+|-
+|align="center"|D121
+|align="center"|MP121(=MP6)
+|align="center"|E0422
+|align="center"|XbaI
+|align="center"|PstI
+|align="center"|3
+|-
+|align="center"|D122
+|align="center"|MP122(=MP8)
+|align="center"|E0840
+|align="center"|XbaI
+|align="center"|PstI
+|align="center"|3
+|}