Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2057 pb
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! rowspan="2"| 707 pb
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| align="center"|SpeI
| align="center"|SpeI
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| align="center"| 2056 pb
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| align="center"| pb
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| align="center"| 708 pb
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! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | D106
 +
! rowspan="4" style="background: #ccccff;" | S03879
|align="center"|1
|align="center"|1
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| align="center"|SpeI
+
| align=center|SpeI
| align="center"|PstI
| align="center"|PstI
| align="center"|BV
| align="center"|BV
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| align="center"|pb
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| align="center"| 2768 pb
| align="center"|14 pb
| align="center"|14 pb
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! rowspan="2"| PstI
! rowspan="2"| PstI
! rowspan="2"|  
! rowspan="2"|  
-
! rowspan="2"| pb
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! rowspan="2"| 2057 pb
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! rowspan="2"| pb
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! rowspan="2"| 725 pb
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| align="center"|SpeI
| align="center"|SpeI
| align="center"|
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-
| align="center"|pb
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| align="center"| 2756 pb
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| align="center"| pb
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| align="center"| 726 pb
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|-
|-
|align="center"|D107
|align="center"|D107
-
|align="center"|MP107
 
|align="center"|C0079
|align="center"|C0079
 +
|align="center"| 1
|align="center"|EcoRI
|align="center"|EcoRI
|align="center"|SpeI
|align="center"|SpeI
-
|align="center"|1
+
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 +
|align="center"|
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|-
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|align="center"|D108
|align="center"|D108

Revision as of 18:06, 24 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enzyme 1 Enzyme 2 Observation Exp Size Matrix Exp Size BioBrick Mea Size Matrix Mea Size BioBrick
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
2
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI 2057 pb 707 pb
3
4 EcoRI SpeI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI 2057 pb 725 pb
3
4 EcoRI SpeI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3
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