Team:Paris/July 25

From 2008.igem.org

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== MiniPreps ==
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== MiniPreps / Digestion ==
*The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
*The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
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{| border="1"
 
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|align="center"|'''Name'''
 
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|align="center"|'''Biobrick'''
 
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|align="center"|'''Description'''
 
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|align="center"|MP100
 
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|align="center"|B0034
 
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|align="center"|
 
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|align="center"|MP116
 
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|align="center"|J23100
 
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|align="center"|
 
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|align="center"|MP120
 
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|align="center"|B0030
 
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|align="center"|
 
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|align="center"|MP121
 
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|align="center"|E0422
 
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|align="center"|
 
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|align="center"|MP122
 
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|align="center"|E0840
 
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|align="center"|
 
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|}
 
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== Digestion ==
 
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===Digestion Mix===
===Digestion Mix===

Revision as of 16:49, 25 July 2008

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Contents

MiniPreps / Digestion

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.

Digestion Mix

10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D116 MP116 J23100 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 1 !
D122 MP122 E0840 XbaI PstI 1 !


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb
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