Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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|align=center|'''Mea Size Matrix'''
|align=center|'''Mea Size Matrix'''
|align=center|'''Mea Size BB'''
|align=center|'''Mea Size BB'''
 +
|align=center|'''Gel'''
 +
|align=center|'''Band'''
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! rowspan="5" style="background: #ccccff;" | D100
! rowspan="5" style="background: #ccccff;" | D100
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! rowspan="2"| not digested
! rowspan="2"| not digested
! rowspan="2"| not digested
! rowspan="2"| not digested
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|align=center| 8
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|align=center| 5-6
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|align=center|2
|align=center|2
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|align=center| 2100 pb  
|align=center| 2100 pb  
|align=center| -
|align=center| -
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|align=center| 1
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|align=center| 2
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|align=center|4
|align=center|4
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! rowspan="2"| 2000 pb
! rowspan="2"| 2000 pb
! rowspan="2"| -
! rowspan="2"| -
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|align=center| 1
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|align=center| 3-4
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|align=center|5
|align=center|5
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|align=center|
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|align=center| 1
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|align=center|
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|align=center| 5-6
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! rowspan="2" style="background: #ccccff;" | D101
! rowspan="2" style="background: #ccccff;" | D101
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! rowspan="2"| 2000 pb
! rowspan="2"| 2000 pb
! rowspan="2"| 900 pb
! rowspan="2"| 900 pb
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|align=center| 1
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|align=center| 7-8
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|align=center|2
|align=center|2
|align=center|
|align=center|
|align=center|
|align=center|
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|align=center| 1
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|align=center| 9-10
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! rowspan="1" style="background: #ccccff;" | D102
! rowspan="1" style="background: #ccccff;" | D102
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|align=center| 2000 pb
|align=center| 2000 pb
|align=center| 900 pb
|align=center| 900 pb
 +
|align=center| 1 & 2
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|align=center| 11-12 & 2-3
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! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | D103
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! rowspan="2"| 2500 pb
! rowspan="2"| 2500 pb
! rowspan="2"| -
! rowspan="2"| -
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|align=center| 2
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|align=center| 4-5
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|align=center|2
|align=center|2
-
|align=center|
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|align=center| 2
-
|align=center|
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|align=center| 6-7
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! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | D104
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! rowspan="2"| 2500 pb
! rowspan="2"| 2500 pb
! rowspan="2"| -
! rowspan="2"| -
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|align=center| 2
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|align=center| 8-9
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|align=center|2
|align=center|2
-
|align=center|
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|align=center| 2
-
|align=center|
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|align=center| 10-11
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! rowspan="4" style="background: #ccccff;" | D105
! rowspan="4" style="background: #ccccff;" | D105
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|align=center| 3000 pb  
|align=center| 3000 pb  
|align=center| -
|align=center| -
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|align=center| 2 & 3
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|align=center| 12 & 2
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|align=center|2
|align=center|2
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! rowspan="2"| 2000 pb  
! rowspan="2"| 2000 pb  
! rowspan="2"| 700 pb
! rowspan="2"| 700 pb
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|align=center| 3
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|align=center| 3-4
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|align=center|3
|align=center|3
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|align=center|
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|align=center| 3
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|align=center|
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|align=center| 5-6
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|align=center|4
|align=center|4
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|align=center| 2000 pb
|align=center| 2000 pb
|align=center| 700 pb
|align=center| 700 pb
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|align=center| 3
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|align=center| 7-8
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! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | D106
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|align=center| 3000 pb
|align=center| 3000 pb
|align=center| -
|align=center| -
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|align=center| 3
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|align=center| 9-10
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|align=center|2
|align=center|2
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! rowspan="2"| 2000 pb
! rowspan="2"| 2000 pb
! rowspan="2"| 700 pb
! rowspan="2"| 700 pb
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|align=center| 3
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|align=center| 11-12
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|align=center|3
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|align=center| 2-3
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|align=center|  2500 pb
|align=center|  2500 pb
|align=center| 700 pb
|align=center| 700 pb
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|align=center| 4
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|align=center| 4-5
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! rowspan="2" style="background: #ccccff;" |D107
! rowspan="2" style="background: #ccccff;" |D107
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|align=center| 6000 pb
|align=center| 6000 pb
|align=center| 1000 pb
|align=center| 1000 pb
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|align=center| 4
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|align=center| 6-7
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|align=center| 2
|align=center| 2
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|align=center| 2500 pb
|align=center| 2500 pb
|align=center| 1000 pb
|align=center| 1000 pb
 +
|align=center| 4
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|align=center| 8-9
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|align=center| 2
|align=center| 2
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|align=center| 4000 pb
|align=center| 4000 pb
|align=center| 1000 pb
|align=center| 1000 pb
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|align=center| 4
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|align=center| 10-11
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|align=center| 2
|align=center| 2
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|align=center| 2000 pb
|align=center| 2000 pb
|align=center| 800 pb
|align=center| 800 pb
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|align=center| 4 & 5
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|align=center| 12 & 2
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|align=center| 2
|align=center| 2
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|align=center| 4000 pb
|align=center| 4000 pb
|align=center| 600 pb
|align=center| 600 pb
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|align=center| 5
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|align=center| 3-4
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Revision as of 11:29, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb not digested not digested 8 5-6
2
3 EcoRI XbaI FV 2076 pb 15 pb 2100 pb - 1 2
4 SpeI PstI BV 2077 pb 14 pb 2000 pb - 1 3-4
5 1 5-6
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 1 7-8
2 1 9-10
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 1 & 2 11-12 & 2-3
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 2500 pb - 2 4-5
2 2 6-7
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 2500 pb - 2 8-9
2 2 10-11
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 3000 pb - 2 & 3 12 & 2
2 XbaI PstI 2057 pb 707 pb 2000 pb 700 pb 3 3-4
3 3 5-6
4 EcoRI SpeI 2056 pb 708 pb 2000 pb 700 pb 3 7-8
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 3000 pb - 3 9-10
2 XbaI PstI 2057 pb 725 pb 2000 pb 700 pb 3 11-12
3 4 2-3
4 EcoRI SpeI 2756 pb 726 pb 2500 pb 700 pb 4 4-5
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 6000 pb 1000 pb 4 6-7
2 XbaI PstI BI 4003 pb 778 pb 5000 pb 100 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2500 pb 1000 pb 4 8-9
2 XbaI PstI BI 4403 pb 745 pb 2500 pb 1000 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 4000 pb 1000 pb 4 10-11
2 XbaI PstI BI 3167 pb 745 pb 3000 pb 1000 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 2000 pb 800 pb 4 & 5 12 & 2
2 XbaI PstI BI 2057 pb 742 pb 2500 pb 1000 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 4000 pb 600 pb 5 3-4
2 XbaI PstI BI 4403 pb 703 pb 4500 pb 700 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb Not realised
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 800 pb
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 3000 pb -
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 6000 - 3000 pb -
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb not digested not digested
2
3 EcoRI XbaI FV 2079 pb 15 pb 1600 pb -
4 SpeI PstI BV 2080 pb 14 pb 3000 - 2000 pb -
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb 3000 pb Not digested
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb 2500 pb Not digested

gel1
gel1
gel2
gel2
gel3
gel3
gel4
gel4
gel5
gel5
gel6
gel5
gel7
gel5
gel8
gel5
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