Team:Paris/July 24

From 2008.igem.org

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1µl of enzyme 2<br>
1µl of enzyme 2<br>
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===list of digestion===
 
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{| border="1"
 
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|align="center"|'''Name'''
 
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|align="center"|'''Miniprep'''
 
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|align="center"|'''Biobrick'''
 
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|align="center"|'''Enzyme 1'''
 
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|align="center"|'''Enzyme 2'''
 
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|align="center"|'''Nber tube'''
 
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|-
 
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|align="center"|D100
 
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|align="center"|MP100
 
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|align="center"|B0034
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D101
 
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|align="center"|MP101
 
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|align="center"|J23101
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D102
 
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|align="center"|MP102
 
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|align="center"|J23109
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D103
 
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|align="center"|MP103
 
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|align="center"|R0079
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D104
 
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|align="center"|MP104
 
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|align="center"|R0040
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D105
 
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|align="center"|MP105
 
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|align="center"|S03154
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D106
 
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|align="center"|MP106
 
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|align="center"|J23101
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D107
 
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|align="center"|MP107
 
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|align="center"|C0079
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D108
 
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|align="center"|MP108
 
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|align="center"|C0179
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D109
 
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|align="center"|MP109
 
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|align="center"|E0030
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D110
 
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|align="center"|MP110
 
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|align="center"|E0040
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D111
 
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|align="center"|MP111
 
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|align="center"|E1010
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D116
 
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|align="center"|MP116
 
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|align="center"|J23100
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D117
 
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|align="center"|MP117
 
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|align="center"|J23107
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D118
 
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|align="center"|MP118
 
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|align="center"|B0015
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|2
 
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|-
 
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|align="center"|D119
 
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|align="center"|MP119
 
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|align="center"|I0500
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D120
 
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|align="center"|MP120
 
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|align="center"|B0030
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D121
 
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|align="center"|MP121
 
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|align="center"|E0422
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|3
 
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|-
 
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|align="center"|D122
 
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|align="center"|MP122
 
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|align="center"|E0840
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|3
 
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|}
 
== Results of digestions : Electrophoresis ==
== Results of digestions : Electrophoresis ==

Revision as of 13:28, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

gel1 gel1 gel2 gel2 gel3 gel3 gel4 gel4


gel5 gel5 gel6 gel6 gel7 gel7 gel8 gel8


Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb not digested not digested 8 5-6
2
3 EcoRI XbaI FV 2076 pb 15 pb 2100 pb - 1 2
4 SpeI PstI BV 2077 pb 14 pb 2000 pb - 1 3-4
5 1 5-6
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 1 7-8
2 1 9-10
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 900 pb 1 & 2 11-12 & 2-3
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 2500 pb - 2 4-5
2 2 6-7
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 2500 pb - 2 8-9
2 2 10-11
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 3000 pb - 2 & 3 12 & 2
2 XbaI PstI 2057 pb 707 pb 2000 pb 700 pb 3 3-4
3 3 5-6
4 EcoRI SpeI 2056 pb 708 pb 2000 pb 700 pb 3 7-8
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 3000 pb - 3 9-10
2 XbaI PstI 2057 pb 725 pb 2000 pb 700 pb 3 11-12
3 4 2-3
4 EcoRI SpeI 2756 pb 726 pb 2500 pb 700 pb 4 4-5
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 6000 pb 1000 pb 4 6-7
2 XbaI PstI BI 4003 pb 778 pb 5000 pb 100 pb 6 9
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2500 pb 1000 pb 4 8-9
2 XbaI PstI BI 4403 pb 745 pb 2500 pb 1000 pb 6 10
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 4000 pb 1000 pb 4 10-11
2 XbaI PstI BI 3167 pb 745 pb 3000 pb 1000 pb 6 11
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 2000 pb 800 pb 4 & 5 12 & 2
2 XbaI PstI BI 2057 pb 742 pb 2500 pb 1000 pb 6 12
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 4000 pb 600 pb 5 3-4
2 XbaI PstI BI 4403 pb 703 pb 4500 pb 700 pb 8 2-3
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb Not realised
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 2000 pb 800 pb 5 5-6
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 3000 pb - 5 7-8
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 6000 - 3000 pb - 5 9-10
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb not digested not digested 7 2
2 7 5-6
3 EcoRI XbaI FV 2079 pb 15 pb 1600 pb - 6 2
4 SpeI PstI BV 2080 pb 14 pb 3000 - 2000 pb - 5 11
5 5 12
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb 3000 pb Not digested 6 3-5
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb 2500 pb Not digested 6 6-8
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