From 2008.igem.org
(Difference between revisions)
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| | |align="center"|MP116 | | |align="center"|MP116 |
| - | |align="center"|J23100 | + | |align="center"|[http://partsregistry.org/Part:BBa_J23100 J23100] |
| - | |align="center"| | + | |align="center"|Strong constitutive promoter in J61002 |
| | |- | | |- |
| | |align="center"|MP117 | | |align="center"|MP117 |
| - | |align="center"|J23107 | + | |align="center"|[http://partsregistry.org/Part:BBa_J23107 J23107] |
| - | |align="center"| | + | |align="center"|Medium constitutive promoter in J61002 |
| | |- | | |- |
| | |align="center"|MP118 | | |align="center"|MP118 |
| - | |align="center"|B0015 | + | |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] |
| - | |align="center"| | + | |align="center"|Double terminator |
| | |- | | |- |
| | |align="center"|MP119 | | |align="center"|MP119 |
| - | |align="center"|I0500 | + | |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] |
| - | |align="center"| | + | |align="center"|AraC pBAD |
| | |- | | |- |
| | |align="center"|MP120 | | |align="center"|MP120 |
| - | |align="center"|B0030 | + | |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] |
| - | |align="center"| | + | |align="center"|Strong RBS (Efficiency = 0,6) |
| | |- | | |- |
| | |align="center"|MP121 | | |align="center"|MP121 |
| - | |align="center"|E0422 | + | |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] |
| - | |align="center"| | + | |align="center"|ECFP (RBS+LVA+Term) |
| | |- | | |- |
| - | |align="center"|MP122 | + | |align="center"| MP122 |
| - | |align="center"|E0840 | + | |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] |
| - | |align="center"| | + | |align="center"|gfp tri-part; strong rbs |
| | + | | |
| | |} | | |} |
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Revision as of 15:01, 26 July 2008
|
← Yesterday ↓ Calendar ↑Tomorrow →
MiniPreps
- The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
| Name
| Biobrick
| Description
|
| MP116
| [http://partsregistry.org/Part:BBa_J23100 J23100]
| Strong constitutive promoter in J61002
|
| MP117
| [http://partsregistry.org/Part:BBa_J23107 J23107]
| Medium constitutive promoter in J61002
|
| MP118
| [http://partsregistry.org/Part:BBa_B0015 B0015]
| Double terminator
|
| MP119
| [http://partsregistry.org/Part:BBa_I0500 I0500]
| AraC pBAD
|
| MP120
| [http://partsregistry.org/Part:BBa_B0030 B0030]
| Strong RBS (Efficiency = 0,6)
|
| MP121
| [http://partsregistry.org/Part:BBa_E0422 E0422]
| ECFP (RBS+LVA+Term)
|
| MP122
| [http://partsregistry.org/Part:BBa_E0840 E0840]
| gfp tri-part; strong rbs
|
|
Digestion
Digestion Mix
10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2
Results of digestions : Electrophoresis
gel1 
gel2 
gel3 
gel4 
gel5 
gel6 
gel7 
gel8 
| Name
| BioBrick
| Tube N°
| Enz 1
| Enz 2
| Obs
| Exp Size Matrix
| Exp Size BB
| Mea Size Matrix
| Mea Size BB
| Gel
| Band
|
| D100
| B0034
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 34 pb
| not digested
| not digested
| 8
| 5-6
|
| 2
|
| 3
| EcoRI
| XbaI
| FV
| 2076 pb
| 15 pb
| 2100 pb
| -
| 1
| 2
|
| 4
| SpeI
| PstI
| BV
| 2077 pb
| 14 pb
| 2000 pb
| -
| 1
| 3-4
|
| 5
| 1
| 5-6
|
| D101
| J23101
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
| 2000 pb
| 900 pb
| 1
| 7-8
|
| 2
| 1
| 9-10
|
| D102
| J23109
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
| 2000 pb
| 900 pb
| 1 & 2
| 11-12 & 2-3
|
| D103
| R0079
| 1
| SpeI
| PstI
| BV
| 2222 pb
| 14 pb
| 2500 pb
| -
| 2
| 4-5
|
| 2
| 2
| 6-7
|
| D104
| R0040
| 1
| SpeI
| PstI
| BV
| 2119 pb
| 14 pb
| 2500 pb
| -
| 2
| 8-9
|
| 2
| 2
| 10-11
|
| D105
| S03154
| 1
| SpeI
| PstI
| BV
| 2750 pb
| 14 pb
| 3000 pb
| -
| 2 & 3
| 12 & 2
|
| 2
| XbaI
| PstI
| BI
| 2057 pb
| 707 pb
| 2000 pb
| 700 pb
| 3
| 3-4
|
| 3
| 3
| 5-6
|
| 4
| EcoRI
| SpeI
| FI
| 2056 pb
| 708 pb
| 2000 pb
| 700 pb
| 3
| 7-8
|
| D106
| S03879
| 1
| SpeI
| PstI
| BV
| 2768 pb
| 14 pb
| 3000 pb
| -
| 3
| 9-10
|
| 2
| XbaI
| PstI
| BI
| 2057 pb
| 725 pb
| 2000 pb
| 700 pb
| 3
| 11-12
|
| 3
| 4
| 2-3
|
| 4
| EcoRI
| SpeI
| FI
| 2756 pb
| 726 pb
| 2500 pb
| 700 pb
| 4
| 4-5
|
| D107
| C0079
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 779 pb
| 6000 pb
| 1000 pb
| 4
| 6-7
|
| 2
| XbaI
| PstI
| BI
| 4003 pb
| 778 pb
| 5000 pb
| 100 pb
| 6
| 9
|
| D108
| C0179
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 746 pb
| 2500 pb
| 1000 pb
| 4
| 8-9
|
| 2
| XbaI
| PstI
| BI
| 4403 pb
| 745 pb
| 2500 pb
| 1000 pb
| 6
| 10
|
| D109
| E0030
| 1
| EcoRI
| SpeI
| FI
| 3166 pb
| 746 pb
| 4000 pb
| 1000 pb
| 4
| 10-11
|
| 2
| XbaI
| PstI
| BI
| 3167 pb
| 745 pb
| 3000 pb
| 1000 pb
| 6
| 11
|
| D110
| E0040
| 1
| EcoRI
| SpeI
| FI
| 2056 pb
| 743 pb
| 2000 pb
| 800 pb
| 4 & 5
| 12 & 2
|
| 2
| XbaI
| PstI
| BI
| 2057 pb
| 742 pb
| 2500 pb
| 1000 pb
| 6
| 12
|
| D111
| E1010
| 1
| EcoRI
| SpeI
| FI
| 4402 pb
| 704 pb
| 4000 pb
| 600 pb
| 5
| 3-4
|
| 2
| XbaI
| PstI
| BI
| 4403 pb
| 703 pb
| 4500 pb
| 700 pb
| 8
| 2-3
|
| D116
| J23100
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
| Not realised
|
|
| D117
| J23107
| 1
| SpeI
| PstI
| BV
| 2100 pb
| 883 pb
| 2000 pb
| 800 pb
| 5
| 5-6
|
| D118
| B0015
| 1
| EcoRI
| XbaI
| FV
| 3303 pb
| 15 pb
| 3000 pb
| -
| 5
| 7-8
|
| D119
| I0500
| 1
| SpeI
| PstI
| FV
| 5621 pb
| 14 pb
| 6000 - 3000 pb
| -
| 5
| 9-10
|
| D120
| B0030
| 1
| XbaI
| PstI
| BI
| 2057 pb
| 37 pb
| not digested
| not digested
| 7
| 2
|
| 2
| 7
| 5-6
|
| 3
| EcoRI
| XbaI
| FV
| 2079 pb
| 15 pb
| 1600 pb
| -
| 6
| 2
|
| 4
| SpeI
| PstI
| BV
| 2080 pb
| 14 pb
| 3000 - 2000 pb
| -
| 5
| 11
|
| 5
| 5
| 12
|
| D121
| E0422
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 939 pb
| 3000 pb
| Not digested
| 6
| 3-5
|
| D122
| E0840
| 1
| XbaI
| PstI
| FV
| 2057 pb
| 900 pb
| 2500 pb
| Not digested
| 6
| 6-8
|
|
"
