Team:Paris/July 27

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Extraction of the DNA

Results of the extraction : Electrophoresis

conditions :

10µl of ladder 1 kb
2µl of extraction added with 2µl of loading Dye 6x
migration ~30min at 100W
Gel 1, 2, 3, 4, 5 = 0.8%
Gel 6 = 1,5%

gel1 gel2 gel3

Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size	Mea Size	Mass (ng)	Gel	Band
D100	B0034	1	XbaI	PstI	BI	2057 pb
		2	XbaI	PstI	BI	2057 pb
		3	EcoRI	XbaI	FV	2076 pb			1	2
		4	SpeI	PstI	BV	2077 pb			1	3
		5	SpeI	PstI	BV	2077 pb			1	4
D101	J23101	1	SpeI	PstI	BV	2100 pb			1	5
D101	J23101	2	SpeI	PstI	BV	2100 pb			1	6
D102	J23109	1	SpeI	PstI	BV	2100 pb			1	7
D102	J23109	2	SpeI	PstI	BV	2100 pb			1	8
D103	R0079	1	SpeI	PstI	BV	2222 pb			2	2
D103	R0079	2	SpeI	PstI	BV	2222 pb			2	3
D104	R0040	1	SpeI	PstI	BV	2119 pb			2	4
D104	R0040	2	SpeI	PstI	BV	2119 pb			2	5
D105	S03154	1	SpeI	PstI	BV	2750 pb			2	6
		2	XbaI	PstI	BI	707 pb			2	7
		3	XbaI	PstI	BI	707 pb			2	8
		4	EcoRI	SpeI	FI	708 pb			3	2
D106	S03879	1	SpeI	PstI	BV	2768 pb			3	3
		2	XbaI	PstI	BI	725 pb			3	4
		3	XbaI	PstI	BI	725 pb			3	5
		4	EcoRI	SpeI	FI	726 pb			3	6
D107	C0079	1	EcoRI	SpeI	FI	779 pb			3	7
D107	C0079	2	XbaI	PstI	BI	778 pb			3	8
D108	C0179	1	EcoRI	SpeI	FI	746 pb
D108	C0179	2	XbaI	PstI	BI	745 pb
D109	E0030	1	EcoRI	SpeI	FI	746 pb
D109	E0030	2	XbaI	PstI	BI	745 pb
D110	E0040	1	EcoRI	SpeI	FI	743 pb
D110	E0040	2	XbaI	PstI	BI	742 pb
D111	E1010	1	EcoRI	SpeI	FI	704 pb
D111	E1010	2	XbaI	PstI	BI	703 pb
D116	J23100	1	SpeI	PstI	BV	2100 pb
D116	J23100	2	SpeI	PstI	BV	2100 pb
D117	J23107	1	SpeI	PstI	BV	2100 pb
D117	J23107	2	SpeI	PstI	BV	2100 pb
D118	B0015	1	EcoRI	XbaI	FV	3303 pb
D118	B0015	2	EcoRI	XbaI	FV	3303 pb
D119	I0500	1	SpeI	PstI	FV	5621 pb
D119	I0500	2	SpeI	PstI	FV	5621 pb
D120	B0030	1	XbaI	PstI	BI	37 pb
		2	XbaI	PstI	BI	37 pb
		3	EcoRI	XbaI	FV	2079 pb
		4	SpeI	PstI	BV	2080 pb
		5	SpeI	PstI	BV	2080 pb
D121	E0422	1	XbaI	PstI	BI	939 pb
		2
		3
D122	E0840	1	XbaI	PstI	BI	900 pb
		2
		3

==> conclusion :

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-=== Results of extraction : Electrophoresis ===
+{{Paris/Calendar_Links|July 25|July 27}}
+== Extraction of the DNA ==
+=== Results of the extraction : Electrophoresis ===
 conditions :
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