Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(→Results of Ligation transformant)
Line 2: Line 2:
-
=== Results of Ligation transformant ===
+
=== Results of the transformation ===
 +
 
{| border="1"  
{| border="1"  
|align="center"|'''Ligation name'''
|align="center"|'''Ligation name'''
-
|align="center"|'''Nb of colonies'''
 
|align="center"|'''Description'''
|align="center"|'''Description'''
 +
|align="center"|'''Antibio'''
 +
|align="center"|'''Nb colonies observed'''
 +
|align="center"|'''Fluorescence'''
 +
|align="center"|'''Comments'''
|-
|-
|align="center"|L100
|align="center"|L100
 +
|align="center"|
 +
rbs TetR - ECFP
 +
<br>D110 (BV) -  D130 (BI) 
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|rbs-TetR <-> ECFP (RBS+LVA+Term)
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L101
|align="center"|L101
 +
|align="center"|
 +
rbs TetR - GFP tripart
 +
<br>D110 (BV) -  D131 (BI) 
 +
|align="center"|
|align="center"|(1)
|align="center"|(1)
-
|align="center"|rbs-TetR <-> GFP tripart+strong rbs
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L102
|align="center"|L102
 +
|align="center"|
 +
Strong rbs - YFP
 +
<br>D129 (BV) -  D118 (BI) 
 +
|align="center"|
|align="center"|+/- 100
|align="center"|+/- 100
-
|align="center"|strong RBS (0,6)<-> YFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L103
|align="center"|L103
 +
|align="center"|
 +
Strong rbs - GFP
 +
<br>D129 (BV) -  D122 (BI) 
 +
|align="center"|
|align="center"|a lot ++  
|align="center"|a lot ++  
-
|align="center"|strong RBS (0,6)<-> GFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L104
|align="center"|L104
 +
|align="center"|
 +
Strong rbs - lasR activator
 +
<br>D129 (BV) -  D114 (BI) 
 +
|align="center"|
|align="center"|a lot ++  
|align="center"|a lot ++  
-
|align="center"|strong RBS (0,6)<-> LasR activator (+LVA)
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L105
|align="center"|L105
 +
|align="center"|
 +
Strong promoter - ECFP
 +
<br>D123 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|a lot ++  
|align="center"|a lot ++  
-
|align="center"|strong constitutive promoter <-> ECFP
+
|align="center"|
 +
|align="center"|OK
|-
|-
|align="center"|L106
|align="center"|L106
 +
|align="center"|
 +
Strong promoter - gfp Tripart
 +
<br>D123 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|a lot ++  
|align="center"|a lot ++  
-
|align="center"|strong constitutive promoter <-> GFP tripart
+
|align="center"|
 +
|align="center"|OK
|-
|-
|align="center"|L107
|align="center"|L107
 +
|align="center"|
 +
Strongest promoter - ECFP
 +
<br>D103 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|a lot ++  
|align="center"|a lot ++  
-
|align="center"|strongest constitutive promoter <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
-
|align="center"|L108
+
|align="center"|L108 n°2 (the right one)
-
|align="center"|+/- 100 (2 dishes!?)
+
|align="center"|
-
|align="center"|strongest constitutive promoter <-> GFP tripart
+
Strong promoter - gfp Tripart
 +
<br>D103 (BV) - D130 (BI)
 +
|align="center"|
 +
|align="center"|+/- 100
 +
|align="center"|
 +
|align="center"|OK
|-
|-
|align="center"|L109
|align="center"|L109
-
|align="center"|+/- 30 or a lot ++ (2 dishes !?)  
+
|align="center"|
-
|align="center"|medium constitutive promoter <-> ECFP
+
Strong promoter - ecfp
 +
<br>D124 (BV) - D130 (BI)
 +
|align="center"|
 +
|align="center"|+/- 100
 +
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L110
|align="center"|L110
 +
|align="center"|
 +
Medium promoter - gfp Tripart
 +
<br>D124 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|medium constitutive promoter <-> gfp tripart
+
|align="center"|
 +
|align="center"|OK
|-
|-
|align="center"|L111
|align="center"|L111
 +
|align="center"|
 +
Weak promoter - ECFP
 +
<br>D104 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|weak constitutive promoter <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L112
|align="center"|L112
 +
|align="center"|
 +
Weak promoter - gfp
 +
<br>D104 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|weak constitutive promoter <-> gfp tripart
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L113
|align="center"|L113
 +
|align="center"|
 +
AracpBAD - ecfp
 +
<br>D126 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|1
|align="center"|1
-
|align="center"|Arac pBAD <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L114
|align="center"|L114
 +
|align="center"|
 +
AracpBAD - gfp tripart
 +
<br>D126 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|2
|align="center"|2
-
|align="center"|Arac pBAD <-> gfp tripart
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L115
|align="center"|L115
 +
|align="center"|
 +
pLas - ECFP
 +
<br>D105 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|pLas <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L116
|align="center"|L116
 +
|align="center"|
 +
pLas - gfp Tripart
 +
<br>D105 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|pLas <-> gfp tripart
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L117
|align="center"|L117
 +
|align="center"|
 +
yfp - Double Terminator
 +
<br>D117 (FI) - D125 (FV)
 +
|align="center"|
|align="center"|+/- 30
|align="center"|+/- 30
-
|align="center"|Double terminator <-> YFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L118
|align="center"|L118
 +
|align="center"|
 +
rfp - Double Terminator
 +
<br>D121 (FI) - D125 (FV)
 +
|align="center"|
|align="center"|+/- 30
|align="center"|+/- 30
-
|align="center"|Double terminator <-> mRFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L119
|align="center"|L119
 +
|align="center"|
 +
lasR activator + LVA - Double Terminator
 +
<br>D113 (FI) - D125 (FV)
 +
|align="center"|
|align="center"|+/- 30
|align="center"|+/- 30
-
|align="center"|Double terminator <-> LasR activator (+LVA)
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L120
|align="center"|L120
 +
|align="center"|
 +
tetR repressible - ECFP
 +
<br>D106 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|tetR repressible promoter <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L121
|align="center"|L121
 +
|align="center"|
 +
Strong promoter - gfp tripart
 +
<br>D106 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|tetR repressible promoter <-> gfp tripart
+
|align="center"|
 +
|align="center"|OK
|-
|-
|align="center"|L122
|align="center"|L122
 +
|align="center"|
 +
RBS-lasI - ecfp
 +
<br>D107 (BV) - D130 (BI)
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|B0034rbs-LasI <-> ECFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L123
|align="center"|L123
 +
|align="center"|
 +
RBS lasI - ecfp
 +
<br>D107 (BV) - D131 (BI)
 +
|align="center"|
|align="center"|1
|align="center"|1
-
|align="center"|B0034rbs-LasI <-> gfp tripart
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L124
|align="center"|L124
 +
|align="center"|
 +
Strongest RBS - rfp
 +
<br>D102 (BV) - D122 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|strongest RBS (1) <-> GFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L125
|align="center"|L125
 +
|align="center"|
 +
Strongest RBS - yfp
 +
<br>D102 (BV) - D118 (BI)
 +
|align="center"|
|align="center"|a lot ++
|align="center"|a lot ++
-
|align="center"|strongest RBS (1) <-> YFP
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L126
|align="center"|L126
 +
|align="center"|
 +
Strongest RBS (1)- LacR activator (+LVA)
 +
<br>D102 (BV) - D118 (BI)
 +
|align="center"|
|align="center"|no dish found!!!
|align="center"|no dish found!!!
-
|align="center"|strongest RBS (1) <->  activator (+LVA)
+
|align="center"|
 +
|align="center"|To do again
|-
|-
|align="center"|L127
|align="center"|L127
 +
|align="center"|
 +
gfp (1)- Double terminator
 +
<br>D119 (FI) - D125 (FV)
 +
|align="center"|
|align="center"|no dish found!!!
|align="center"|no dish found!!!
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|To do again
 +
|-
 +
|align="center"|'''Controls'''
 +
|align="center"|
 +
|align="center"|
 +
|align="center"|
 +
|align="center"|
 +
|align="center"|
|-
|-
-
|align="center"|'''C1'''
+
|align="center"|C1
 +
|align="center"|
 +
D110
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|D110
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C2'''
+
|align="center"|C2
 +
|align="center"|
 +
D129
 +
|align="center"|
|align="center"|5
|align="center"|5
-
|align="center"|D129
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C3'''
+
|align="center"|C3
 +
|align="center"|
 +
D123
 +
|align="center"|
|align="center"|+/- 100
|align="center"|+/- 100
-
|align="center"|D123
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C4'''
+
|align="center"|C4
 +
|align="center"|
 +
D103
 +
|align="center"|
|align="center"|+/- 20
|align="center"|+/- 20
-
|align="center"|D103
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C5'''
+
|align="center"|C5
 +
|align="center"|
 +
D124
 +
|align="center"|
|align="center"|+/- 100
|align="center"|+/- 100
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C6'''
+
|align="center"|C6
 +
|align="center"|
 +
D104
 +
|align="center"|
|align="center"|+/- 100
|align="center"|+/- 100
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C7'''
+
|align="center"|C7
 +
|align="center"|
 +
D126
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C8'''
+
|align="center"|C8
 +
|align="center"|
 +
D105
 +
|align="center"|
|align="center"|+/- 20
|align="center"|+/- 20
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C9'''
+
|align="center"|C9
 +
|align="center"|
 +
D125
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C10'''
+
|align="center"|C10
 +
|align="center"|
 +
D106
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C11'''
+
|align="center"|C11
 +
|align="center"|
 +
D107
 +
|align="center"|
|align="center"|0
|align="center"|0
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''C12'''
+
|align="center"|C12
 +
|align="center"|
 +
D102
 +
|align="center"|
|align="center"|+/- 10
|align="center"|+/- 10
-
|align="center"|Double terminator <-> GFP
+
|align="center"|
 +
|align="center"|-
|-
|-
-
|align="center"|'''Positive control'''
+
|align="center"|Positive control
 +
|align="center"|
 +
puc19
|align="center"|155 (transformation efficiency:1.5*10^7/ug)
|align="center"|155 (transformation efficiency:1.5*10^7/ug)
 +
|align="center"|
 +
|align="center"|
|align="center"|-
|align="center"|-
|}
|}

Revision as of 18:04, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →


Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

0 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

(1) To do again
L102

Strong rbs - YFP
D129 (BV) - D118 (BI)

+/- 100 To do again
L103

Strong rbs - GFP
D129 (BV) - D122 (BI)

a lot ++ To do again
L104

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

a lot ++ To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

a lot ++ OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

a lot ++ OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

a lot ++ To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

+/- 100 OK
L109

Strong promoter - ecfp
D124 (BV) - D130 (BI)

+/- 100 To do again
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

a lot ++ OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

a lot ++ To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

a lot ++ To do again
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

1 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

2 To do again
L115

pLas - ECFP
D105 (BV) - D130 (BI)

a lot ++ To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

a lot ++ To do again
L117

yfp - Double Terminator
D117 (FI) - D125 (FV)

+/- 30 To do again
L118

rfp - Double Terminator
D121 (FI) - D125 (FV)

+/- 30 To do again
L119

lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)

+/- 30 To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

a lot ++ To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

a lot ++ OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

0 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

1 To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

a lot ++ To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

a lot ++ To do again
L126

Strongest RBS (1)- LacR activator (+LVA)
D102 (BV) - D118 (BI)

no dish found!!! To do again
L127

gfp (1)- Double terminator
D119 (FI) - D125 (FV)

no dish found!!! To do again
Controls
C1

D110

0 -
C2

D129

5 -
C3

D123

+/- 100 -
C4

D103

+/- 20 -
C5

D124

+/- 100 -
C6

D104

+/- 100 -
C7

D126

0 -
C8

D105

+/- 20 -
C9

D125

0 -
C10

D106

0 -
C11

D107

0 -
C12

D102

+/- 10 -
Positive control

puc19

155 (transformation efficiency:1.5*10^7/ug) -
Retrieved from "http://2008.igem.org/Team:Paris/July_31"