Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)

Revision as of 17:51, 4 August 2008

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)		0		To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)		(1)		To do again
L102	Strong rbs - YFP D129 (BV) - D118 (BI)		+/- 100		To do again
L103	Strong rbs - GFP D129 (BV) - D122 (BI)		a lot ++		To do again
L104	Strong rbs - lasR activator D129 (BV) - D114 (BI)		a lot ++		To do again
L105	Strong promoter - ECFP D123 (BV) - D130 (BI)		a lot ++		OK
L106	Strong promoter - gfp Tripart D123 (BV) - D131 (BI)		a lot ++		OK
L107	Strongest promoter - ECFP D103 (BV) - D131 (BI)		a lot ++		To do again
L108 n°2 (the right one)	Strong promoter - gfp Tripart D103 (BV) - D130 (BI)		+/- 100		OK
L109	Strong promoter - ecfp D124 (BV) - D130 (BI)		+/- 100		OK
L110	Medium promoter - gfp Tripart D124 (BV) - D131 (BI)		a lot ++		OK
L111	Weak promoter - ECFP D104 (BV) - D130 (BI)		a lot ++		To do again
L112	Weak promoter - gfp D104 (BV) - D131 (BI)		a lot ++		To do again
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)		1		To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)		2		To do again
L115	pLas - ECFP D105 (BV) - D130 (BI)		a lot ++		To do again
L116	pLas - gfp Tripart D105 (BV) - D131 (BI)		a lot ++		To do again
L117	yfp - Double Terminator D117 (FI) - D125 (FV)		+/- 30		To do again
L118	rfp - Double Terminator D121 (FI) - D125 (FV)		+/- 30		To do again
L119	lasR activator + LVA - Double Terminator D113 (FI) - D125 (FV)		+/- 30		To do again
L120	tetR repressible - ECFP D106 (BV) - D130 (BI)		a lot ++		To do again
L121	Strong promoter - gfp tripart D106 (BV) - D130 (BI)		a lot ++		OK
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)		0		To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)		1		To do again
L124	Strongest RBS - rfp D102 (BV) - D122 (BI)		a lot ++		To do again
L125	Strongest RBS - yfp D102 (BV) - D118 (BI)		a lot ++		To do again
L126	Strongest RBS (1)- LacR activator (+LVA) D102 (BV) - D118 (BI)		no dish found!!!		To do again
L127	gfp (1)- Double terminator D119 (FI) - D125 (FV)		no dish found!!!		To do again

Results of the transformation (bis-the good one to leave in august 2)

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)	Amp	608		To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)	Amp	> 4000	GFP positive	OK
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)	Kana	9		To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)	Kana	9		To do again
L120	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)	Amp	74		To do again
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)	Amp	4		To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)	Amp	> 4000		To do again
L126	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	Amp	944		To do again

@@ Line 13: / Line 13: @@
 |-
 |align="center"|L100
-|align="center"|rbs TetR - ECFP
+|align="center"|
+rbs TetR - ECFP
 <br>D110 (BV) -  D130 (BI)
 |align="center"|
@@ Line 21: / Line 22: @@
 |-
 |align="center"|L101
-|align="center"|rbs TetR - GFP tripart
+|align="center"|
+rbs TetR - GFP tripart
 <br>D110 (BV) -  D131 (BI)
 |align="center"|
@@ Line 29: / Line 31: @@
 |-
 |align="center"|L102
-|align="center"|Strong rbs - YFP
+|align="center"|
+Strong rbs - YFP
 <br>D129 (BV) -  D118 (BI)
 |align="center"|
@@ Line 37: / Line 40: @@
 |-
 |align="center"|L103
-|align="center"|Strong rbs - GFP
+|align="center"|
+Strong rbs - GFP
 <br>D129 (BV) -  D122 (BI)
 |align="center"|
@@ Line 45: / Line 49: @@
 |-
 |align="center"|L104
-|align="center"|Strong rbs - lasR activator
+|align="center"|
+Strong rbs - lasR activator
 <br>D129 (BV) -  D114 (BI)
 |align="center"|
@@ Line 88: / Line 93: @@
 |align="center"|OK
 |-
-|align="center"|L109 to complete
+|align="center"|L109
 |align="center"|
 Strong promoter -  ecfp