Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(→Results of the transformation : Fluorescence)
(→Results of the transformation : Fluorescence)
Line 10: Line 10:
|align="center"|'''Fluorescence'''
|align="center"|'''Fluorescence'''
|align="center"|'''Comments'''
|align="center"|'''Comments'''
-
|-
 
-
|align="center"|L100
 
-
|align="center"|
 
-
rbs TetR - ECFP
 
-
<br>D110 (BV) -  D130 (BI) 
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
|align="center"|L102
|align="center"|L102
Line 22: Line 15:
Strong rbs - YFP
Strong rbs - YFP
<br>D129 (BV) -  D118 (BI)   
<br>D129 (BV) -  D118 (BI)   
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L103
|align="center"|L103
Line 29: Line 22:
Strong rbs - GFP
Strong rbs - GFP
<br>D129 (BV) -  D122 (BI)   
<br>D129 (BV) -  D122 (BI)   
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L104
|align="center"|L104
Line 36: Line 29:
Strong rbs - lasR activator
Strong rbs - lasR activator
<br>D129 (BV) -  D114 (BI)   
<br>D129 (BV) -  D114 (BI)   
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L105
|align="center"|L105
Line 43: Line 36:
Strong promoter - ECFP
Strong promoter - ECFP
<br>D123 (BV) - D130 (BI)
<br>D123 (BV) - D130 (BI)
-
|align="center"|
+
|align="center"|Yes
-
|align="center"|OK
+
|align="center"|OK (some others red because of the promoter)
|-
|-
|align="center"|L106
|align="center"|L106
Line 50: Line 43:
Strong promoter - gfp Tripart
Strong promoter - gfp Tripart
<br>D123 (BV) - D131 (BI)
<br>D123 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|Yes (some others red because of the promoter)
|align="center"|OK
|align="center"|OK
|-
|-
Line 57: Line 50:
Strongest promoter - ECFP
Strongest promoter - ECFP
<br>D103 (BV) - D131 (BI)
<br>D103 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|Yes (some others red because of the promoter)
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L108 n°2 (the right one)
|align="center"|L108 n°2 (the right one)
Line 64: Line 57:
Strong promoter -  gfp Tripart
Strong promoter -  gfp Tripart
<br>D103 (BV) - D130 (BI)
<br>D103 (BV) - D130 (BI)
-
|align="center"|
+
|align="center"|Yes (some others red because of the promoter)
|align="center"|OK
|align="center"|OK
-
|-
 
-
|align="center"|L109
 
-
|align="center"|
 
-
Strong promoter -  ecfp
 
-
<br>D124 (BV) - D130 (BI)
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
|align="center"|L110
|align="center"|L110
Line 78: Line 64:
Medium promoter - gfp Tripart
Medium promoter - gfp Tripart
<br>D124 (BV) - D131 (BI)
<br>D124 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|Yes (some others red because of the promoter)
|align="center"|OK
|align="center"|OK
|-
|-
Line 85: Line 71:
Weak promoter - ECFP
Weak promoter - ECFP
<br>D104 (BV) - D130 (BI)  
<br>D104 (BV) - D130 (BI)  
-
|align="center"|
+
|align="center"|No
|align="center"|To do again
|align="center"|To do again
|-
|-
Line 92: Line 78:
Weak promoter - gfp
Weak promoter - gfp
<br>D104 (BV) - D131 (BI)
<br>D104 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L113
+
-
|align="center"|
+
-
AracpBAD - ecfp
+
-
<br>D126 (BV) - D130 (BI)
+
-
|align="center"|
+
-
|align="center"|To do again
+
-
|-
+
-
|align="center"|L114
+
-
|align="center"|
+
-
AracpBAD - gfp tripart
+
-
<br>D126 (BV) - D131 (BI)
+
-
|align="center"|
+
|align="center"|To do again
|align="center"|To do again
|-
|-
Line 112: Line 84:
|align="center"|
|align="center"|
pLas - ECFP
pLas - ECFP
-
|align="center"|
+
<br> D105 (BV)- D130 (BI)
-
|align="center"|To do again
+
|align="center"|No
 +
|align="center"|OK
|-
|-
|align="center"|L116
|align="center"|L116
Line 119: Line 92:
pLas - gfp Tripart
pLas - gfp Tripart
<br>D105 (BV) - D131 (BI)
<br>D105 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|1 green
|align="center"|To do again
|align="center"|To do again
|-
|-
Line 126: Line 99:
yfp - Double Terminator
yfp - Double Terminator
<br>D117 (FI) - D125 (FV)
<br>D117 (FI) - D125 (FV)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L118
|align="center"|L118
Line 133: Line 106:
rfp - Double Terminator
rfp - Double Terminator
<br>D121 (FI) - D125 (FV)
<br>D121 (FI) - D125 (FV)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L119
|align="center"|L119
Line 140: Line 113:
lasR activator + LVA - Double Terminator
lasR activator + LVA - Double Terminator
<br>D113 (FI) - D125 (FV)
<br>D113 (FI) - D125 (FV)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L120
|align="center"|L120
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tetR repressible - ECFP
tetR repressible - ECFP
<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
-
|align="center"|
+
|align="center"|No
|align="center"|To do again
|align="center"|To do again
|-
|-
Line 154: Line 127:
Strong promoter - gfp tripart
Strong promoter - gfp tripart
<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
-
|align="center"|
+
|align="center"|Yes (some others red because of the promoter)
|align="center"|OK
|align="center"|OK
-
|-
 
-
|align="center"|L122
 
-
|align="center"|
 
-
RBS-lasI - ecfp
 
-
<br>D107 (BV) - D130 (BI)
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
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|align="center"|L123
 
-
|align="center"|
 
-
RBS lasI - ecfp
 
-
<br>D107 (BV) - D131 (BI)
 
-
|align="center"|
 
-
|align="center"|To do again
 
|-
|-
|align="center"|L124
|align="center"|L124
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Strongest RBS - rfp
Strongest RBS - rfp
<br>D102 (BV) - D122 (BI)
<br>D102 (BV) - D122 (BI)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
|align="center"|L125
|align="center"|L125
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Strongest RBS - yfp
Strongest RBS - yfp
<br>D102 (BV) - D118 (BI)
<br>D102 (BV) - D118 (BI)
-
|align="center"|
+
|align="center"|No
-
|align="center"|To do again
+
|align="center"|OK
|-
|-
-
|align="center"|L126
 
-
|align="center"|
 
-
Strongest RBS (1)- LacR activator (+LVA)
 
-
<br>D102 (BV) - D118 (BI)
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
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|align="center"|L127
 
-
|align="center"|
 
-
gfp (1)- Double terminator
 
-
<br>D119 (FI) - D125 (FV)
 
-
|align="center"|
 
-
|align="center"|To do again
 
-
|-
 
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|align="center"|'''Controls'''
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|-
 
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|align="center"|C1
 
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|align="center"|
 
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D110
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|C2
 
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|align="center"|
 
-
D129
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|C3
 
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|align="center"|
 
-
D123
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C4
 
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|align="center"|
 
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D103
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C5
 
-
|align="center"|
 
-
D124
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|C6
 
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|align="center"|
 
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D104
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C7
 
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|align="center"|
 
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D126
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C8
 
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|align="center"|
 
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D105
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|C9
 
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|align="center"|
 
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D125
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|C10
 
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|align="center"|
 
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D106
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C11
 
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|align="center"|
 
-
D107
 
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|align="center"|
 
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|align="center"|-
 
-
|-
 
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|align="center"|C12
 
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|align="center"|
 
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D102
 
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|align="center"|
 
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|align="center"|-
 
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|-
 
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|align="center"|Positive control
 
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|align="center"|
 
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puc19
 
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|align="center"|
 
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|align="center"|-
 
|}
|}

Revision as of 09:47, 5 August 2008

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Results of the transformation : Fluorescence

Ligation name Description Fluorescence Comments
L102

Strong rbs - YFP
D129 (BV) - D118 (BI)

No OK
L103

Strong rbs - GFP
D129 (BV) - D122 (BI)

No OK
L104

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

No OK
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

Yes OK (some others red because of the promoter)
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

Yes (some others red because of the promoter) OK
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

Yes (some others red because of the promoter) OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

No To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

No To do again
L115

pLas - ECFP
D105 (BV)- D130 (BI)

No OK
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

1 green To do again
L117

yfp - Double Terminator
D117 (FI) - D125 (FV)

No OK
L118

rfp - Double Terminator
D121 (FI) - D125 (FV)

No OK
L119

lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)

No OK
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

No To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

Yes (some others red because of the promoter) OK
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

No OK
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

No OK
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