| Ligation name
| Description
| Fluorescence
| Comments
|
| L102
|
Strong rbs - YFP
D129 (BV) - D118 (BI)
| No
| OK
|
| L103
|
Strong rbs - GFP
D129 (BV) - D122 (BI)
| No
| OK
|
| L104
|
Strong rbs - lasR activator
D129 (BV) - D114 (BI)
| No
| OK
|
| L105
|
Strong promoter - ECFP
D123 (BV) - D130 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L106
|
Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L107
|
Strongest promoter - ECFP
D103 (BV) - D131 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L108 n°2 (the right one)
|
Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L110
|
Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L111
|
Weak promoter - ECFP
D104 (BV) - D130 (BI)
| No
| To do again
|
| L112
|
Weak promoter - gfp
D104 (BV) - D131 (BI)
| No
| To do again
|
| L115
|
pLas - ECFP
D105 (BV)- D130 (BI)
| No
| OK
|
| L116
|
pLas - gfp Tripart
D105 (BV) - D131 (BI)
| 1 green
| To do again
|
| L117
|
yfp - Double Terminator
D117 (FI) - D125 (FV)
| No
| OK
|
| L118
|
rfp - Double Terminator
D121 (FI) - D125 (FV)
| No
| OK
|
| L119
|
lasR activator + LVA - Double Terminator
D113 (FI) - D125 (FV)
| No
| OK
|
| L120
|
tetR repressible - ECFP
D106 (BV) - D130 (BI)
| No
| To do again
|
| L121
|
Strong promoter - gfp tripart
D106 (BV) - D130 (BI)
| Yes (some others red because of the promoter)
| OK
|
| L124
|
Strongest RBS - rfp
D102 (BV) - D122 (BI)
| No
| OK
|
| L125
|
Strongest RBS - yfp
D102 (BV) - D118 (BI)
| No
| OK
|
We tried again the following ligations :
L100, L101, L109, L113, L114, L120, L122, L123, L126, L127
"
