Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)

Revision as of 16:18, 6 August 2008

Transformation of 1st August

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)	Amp	608		To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)	Amp	> 4000	GFP positive	OK
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)	Kana	9		To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)	Kana	9		To do again
L120	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)	Amp	74		To do again
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)	Amp	4		To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)	Amp	> 4000		To do again
L126	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	Amp	944		To do again

@@ Line 2: / Line 2: @@
+== Transformation of 1st August ==
 === Results of the transformation ===
@@ Line 15: / Line 17: @@
 |-
 |align="center"|L100
-|align="center"|
+|align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
-rbs TetR - ECFP
-<br>D110 (BV) -  D130 (BI)
 |align="center"|Amp
 |align="center"|608
@@ Line 24: / Line 24: @@
 |-
 |align="center"|L101
-|align="center"|
+|align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI)
-rbs TetR - GFP tripart
-<br>D110 (BV) -  D131 (BI)
 |align="center"|Amp
 |align="center"|> 4000
@@ Line 33: / Line 31: @@
 |-
 |align="center"|L113
-|align="center"|
+|align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI)
-AracpBAD - ecfp
-<br>D126 (BV) - D130 (BI)
 |align="center"|Kana
 |align="center"|9
@@ Line 42: / Line 38: @@
 |-
 |align="center"|L114
-|align="center"|
+|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
-AracpBAD - gfp tripart
-<br>D126 (BV) - D131 (BI)
 |align="center"|Kana
 |align="center"|9
@@ Line 51: / Line 45: @@
 |-
 |align="center"|L120
-|align="center"|
+|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
-tetR repressible promoter - ECFP
-<br>D106 (BV) - D130 (BI)
 |align="center"|Amp
 |align="center"|74
@@ Line 60: / Line 52: @@
 |-
 |align="center"|L122
-|align="center"|
+|align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI)
-RBS-lasI - ecfp
-<br>D107 (BV) - D130 (BI)
 |align="center"|Amp
 |align="center"|4
@@ Line 69: / Line 59: @@
 |-
 |align="center"|L123
-|align="center"|
+|align="center"|RBS lasI - ecfp<br>D107 (BV) - D131 (BI)
-RBS lasI - ecfp
-<br>D107 (BV) - D131 (BI)
 |align="center"|Amp
 |align="center"|> 4000
@@ Line 78: / Line 66: @@
 |-
 |align="center"|L126
-|align="center"|
+|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
-Strongest RBS (1)- LasR activator (+LVA)
-<br>D102 (BV) - D114 (BI)
 |align="center"|Amp
 |align="center"|944