Team:Paris/August 7
From 2008.igem.org
(Difference between revisions)
(→Results of the PCR we did last night) |
(→Results of the PCR we did last night) |
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- | {|- | + | {|- align = "center" | border="1" |
- | | | + | |'''Name''' |
- | + | |'''Promotor''' | |
|align="center"|'''Gel''' | |align="center"|'''Gel''' | ||
|align="center"|'''Band''' | |align="center"|'''Band''' | ||
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|align="center"|pFlgA | |align="center"|pFlgA | ||
|align="center"|1 | |align="center"|1 | ||
- | |align="center"|2 | + | |align="center"|2 |
|style="background: #cbff7B"|<center>261 pb</center> | |style="background: #cbff7B"|<center>261 pb</center> | ||
|align="center"|300 pb | |align="center"|300 pb | ||
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|align="center"|pFlgB | |align="center"|pFlgB | ||
|align="center"|1 | |align="center"|1 | ||
- | |align="center"| | + | |align="center"|3 |
|style="background: #cbff7B"|<center>261 pb</center> | |style="background: #cbff7B"|<center>261 pb</center> | ||
|align="center"|300 pb | |align="center"|300 pb | ||
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|align="center"|PCR_126 | |align="center"|PCR_126 | ||
|align="center"|pFlhB | |align="center"|pFlhB | ||
- | |align="center"| | + | |align="center"|1 |
- | |align="center"| | + | |align="center"|4 |
|style="background: #cbff7B"|<center>260 pb</center> | |style="background: #cbff7B"|<center>260 pb</center> | ||
|align="center"|300 pb | |align="center"|300 pb | ||
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|align="center"|PCR_127 | |align="center"|PCR_127 | ||
|align="center"|pFlhDC | |align="center"|pFlhDC | ||
- | |align="center"| | + | |align="center"|2 |
- | |align="center"| | + | |align="center"|2 to 13 |
|style="background: #ff6d73"|<center>446 pb</center> | |style="background: #ff6d73"|<center>446 pb</center> | ||
- | |align="center"| | + | |align="center"|100 pb |
|} | |} | ||
Revision as of 18:45, 7 August 2008
Result of the isolation of coloniesE0240 and pSB3K3E0240 and pSB3K3 are ok : there is a lot of single colonies S120 and S121S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp.
Results of the PCR we did last night
TransformationsProtocolUse of TOP10 chemically competentcells
List of the Ligation Transformation
PCR Screening of Ligation Transformants of 1st AugustUse of 8 clones of Ligation transformants for screening PCR
Protocol of screening PCR
Conditions of electrophoresis
Results
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