Team:Paris/August 11

From 2008.igem.org

(Difference between revisions)
(Culture of ligation transformants)
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{|| Border="1"
{|| Border="1"
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|- style="text-align: center;"|'''Ligation'''
+
|- style="text-align: center;"
 +
|'''Ligation'''
| colspan="4"|L128
| colspan="4"|L128
| colspan="4"|L129
| colspan="4"|L129
| colspan="4"|L130
| colspan="4"|L130
-
|- style="text-align: center;"|'''Name'''
+
|- style="text-align: center;"
 +
|'''Name'''
| colspan="4"| pFlgA
| colspan="4"| pFlgA
| colspan="4"| pFlgB
| colspan="4"| pFlgB
| colspan="4"|pFlhB
| colspan="4"|pFlhB
-
|- style="text-align: center;"|'''Clone N°'''
+
|- style="text-align: center;"
 +
|'''Clone N°'''
|1
|1
|2
|2
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|7
|7
|8
|8
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|- style="text-align: center;"|'''Red fluorescence'''
+
|- style="text-align: center;"
 +
|'''Red fluorescence'''
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes

Revision as of 18:00, 11 August 2008

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Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
  • 37°C overnight
Ligation L128 L129 L130
Name pFlgA pFlgB pFlhB
Clone N° 1 2 3 4 1 2 6 7 1 2 7 8
Red fluorescence yes yes no no yes yes no no yes no no no