Team:Paris/August 12

From 2008.igem.org

(Difference between revisions)
(Glycerol Stocks)
(Minipreps: Plasmid extraction)
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|'''Description'''
|'''Description'''
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP144
+
|rowspan="4" |MP144
|L128.1
|L128.1
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP145
 
|L128.2
|L128.2
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP146
 
|L128.3
|L128.3
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP147
 
|L128.4
|L128.4
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP148
+
|rowspan="4" |MP145
|L129.1
|L129.1
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP149
 
|L129.2
|L129.2
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP150
 
|L129.6
|L129.6
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP151
 
|L129.7
|L129.7
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP152
+
|rowspan="4" |MP146
|L130.1
|L130.1
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP153
 
|L130.2
|L130.2
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP154
 
|L130.7
|L130.7
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|- style="text-align: center;"
|- style="text-align: center;"
-
|MP155
 
|L130.8
|L130.8
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|}
|}
-
 
==Glycerol Stocks==
==Glycerol Stocks==

Revision as of 17:22, 12 August 2008

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Contents

Minipreps: Plasmid extraction

  • Protocol (see # 3) Experiments done by QIAcube
  • List of Minipreps
Name Ligation Biobricks Description
MP144 L128.1 pFlgA-RFP
D132 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L128.2 pFlgA-RFP
D132 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L128.3 pFlgA-RFP
D132 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L128.4 pFlgA-RFP
D132 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
MP145 L129.1 pFlgB-RFP
D133 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L129.2 pFlgB-RFP
D133 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L129.6 pFlgB-RFP
D133 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L129.7 pFlgB-RFP
D133 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
MP146 L130.1 pFlhB-RFP
D134 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L130.2 pFlhB-RFP
D134 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L130.7 pFlhB-RFP
D134 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
L130.8 pFlhB-RFP
D134 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png

Glycerol Stocks

  • List of Stocks
Strain Ligation Biobricks Description
S143.1 L128.1 pFlgA-RFP
D132 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
S143.2 L128.2
S143.3 L128.3
S143.4 L128.4
S144.1 L129.1 pFlgB-RFP
D133 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
S144.2 L129.2
S144.6 L129.6
S144.7 L129.7
S145.1 L130.1 pFlhB-RFP
D134 (FI) - D136 (FV)
Part icon regulatory.pngPart icon reporter.png
S145.2 L130.2
S145.7 L130.7
S145.8 L130.8

Results of the transformations we did yesterday

Concentration of the MiniPreps

Miniprep Concentration (µg/mL) Ratio 260/280
MP144 127 1.67
MP145 166 1.66
MP146 163 1.57
MP147 154 1.63
MP148 136 1.67
MP149 151 1.63
MP150 163 1.67
MP151 188 1.57
MP152 298 1.70
MP153 142 1.64
MP154 170 1.52
MP155 143 1.55