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Team:Paris/August 12
From 2008.igem.org
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| + | ==New PCR screening with the right pimers== | ||
| + | |||
| + | Transformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers (O18 and O19). | ||
| + | *Template : bacteria from glycerol stock. | ||
| + | *positive control: J61002 (with ptet-mRFP) | ||
| + | *negative control: no template | ||
| + | ===Electrophoresis=== | ||
| + | *1% agarose gel | ||
| + | *10 µL of each sample loaded | ||
| + | |||
| + | {|border="1" | ||
| + | |align="center"|'''well n°''' | ||
| + | |align="center"|1 | ||
| + | |align="center"|2 | ||
| + | |align="center"|3 | ||
| + | |align="center"|4 | ||
| + | |align="center"|5 | ||
| + | |align="center"|6 | ||
| + | |align="center"|7 | ||
| + | |align="center"|8 | ||
| + | |align="center"|9 | ||
| + | |align="center"|10 | ||
| + | |align="center"|11 | ||
| + | |align="center"|12 | ||
| + | |align="center"|13 | ||
| + | |align="center"|14 | ||
| + | |align="center"|15 | ||
| + | |align="center"|16 | ||
| + | |- | ||
| + | |align="center"|'''sample''' | ||
| + | |align="center"|1 kb DNA ladder | ||
| + | |align="center"|positive control | ||
| + | |align="center"|negative control | ||
| + | |colspan="4"|L128 (pFlgA) | ||
| + | |colspan="4"|L129 (pFlgB) | ||
| + | |colspan="4"|L130 (pFlhB) | ||
| + | |align="center"|100 bp DNA ladder | ||
| + | |- | ||
| + | |align="center"|'''expected size''' | ||
| + | |align="center"|ladder | ||
| + | |align="center"|1161 bp | ||
| + | |align="center"|0 bp | ||
| + | |colspan="4"|1339 bp | ||
| + | |colspan="4"|1339 bp | ||
| + | |colspan="4"|1338 bp | ||
| + | |align="center"|ladder | ||
|} | |} | ||
Revision as of 09:17, 13 August 2008
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Minipreps: Plasmid extraction
Glycerol Stocks
Results of the transformations we did yesterdayConcentration of the MiniPreps
New PCR screening with the right pimersTransformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers (O18 and O19).
Electrophoresis
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