Team:Paris/August 13

(Difference between revisions)

Revision as of 18:06, 13 August 2008

Name	Ligation	Biobricks	Description
MP147.1	L100.1	rbs TetR - ECFP D110 (BV) - D130 (BI)
MP147.2	L100.2
MP147.3	L100.3
MP148.1	L101.1	rbs TetR - GFP tripart D110 (BV) - D131 (BI)
MP148.2	L101.2
MP148.3	L101.3
MP149.1	L114.1	AracpBAD - gfp tripart D126 (BV) - D131 (BI)
MP149.2	L114.2
MP149.3	L114.3
MP150.1	L120.1	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)
MP150.2	L120.2
MP150.3	L120.3
MP151.1	L122.1	RBS-lasI - ECFP D107 (BV) - D130 (BI)
MP152.1	L123.1	RBS lasI - ECFP D107 (BV) - D131 (BI)
MP151.2	L123.2
MP152.3	L123.3
MP153.1	L126.1	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)
MP153.2	L126.2
MP153.3	L126.3
MP154.1	L132.1
MP154.2	L132.2
MP154.3	L132.3
MP155.1	L133.1
MP156.1	L134.1
MP156.2	L134.2
MP156.3	L134.3
MP157.1	L138.1

Strain	Ligation	Biobricks	Description
S146.1	L100.1	rbs TetR - ECFP D110 (BV) - D130 (BI)
S146.2	L100.2
S146.3	L100.3
S147.1	L101.1	rbs TetR - GFP tripart D110 (BV) - D131 (BI)
S147.2	L101.2
S147.3	L101.3
S148.1	L114.1	AracpBAD - gfp tripart D126 (BV) - D131 (BI)
S148.2	L114.2
S148.3	L114.3
S149.1	L120.1	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)
S149.2	L120.2
S149.3	L120.3
S150.1	L122.1	RBS-lasI - ECFP D107 (BV) - D130 (BI)
S151.1	L123.1	RBS lasI - ECFP D107 (BV) - D131 (BI)
S152.2	L123.2
S152.3	L123.3
S152.1	L126.1	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)

4 more transformants of L130 (pFlhB into J61002) are screened by PCR.

@@ Line 14: / Line 14: @@
 |'''Vol MP (µL)'''
 |'''Vol H2O (µL)'''
+|'''n° tube'''
 |-
 |MP144.3
@@ Line 20: / Line 21: @@
 |3.68
 |10.32
+|AD1
 |-
 |MP144.4
@@ Line 26: / Line 28: @@
 |3.90
 |10.10
+|AD2
 |-
 |MP145.6
@@ Line 32: / Line 35: @@
 |3.68
 |10.32
+|AD3
 |-
 |MP145.7
@@ Line 38: / Line 42: @@
 |3.19
 |10.81
+|AD4
 |-
 |MP146.1
@@ Line 44: / Line 49: @@
 |2.01
 |11.99
+|AD5
 |-
 |MP146.2
@@ Line 50: / Line 56: @@
 |4.23
 |9.77
+|AD6
 |}
 == Minipreps: Plasmid extraction==