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Team:Paris/August 15
From 2008.igem.org
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(→Transformation of the ligations we did yesterday) |
(→Ligation) |
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| Line 38: | Line 38: | ||
|Vector | |Vector | ||
|- | |- | ||
| - | | | + | |L150 |
|D146 (strongest rbs-TetR-GFP tripart) | |D146 (strongest rbs-TetR-GFP tripart) | ||
|D105 (pLas) | |D105 (pLas) | ||
|- | |- | ||
| - | | | + | |L151 |
|D147 (strongest rbs-LasR activator with LVA) | |D147 (strongest rbs-LasR activator with LVA) | ||
|D125 (Double terminator) | |D125 (Double terminator) | ||
| + | |- | ||
| + | |Control 1 | ||
| + | | / | ||
| + | |D105 | ||
| + | |- | ||
| + | |Control 2 | ||
| + | | / | ||
| + | |D125 | ||
| + | |- | ||
| + | |Positive Control | ||
| + | |Puc19 | ||
| + | | / | ||
|} | |} | ||
Revision as of 13:22, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells standard protocol. DigestionMeasurement of concentration of minipreps
DigestionLigation
Analysis of yesterday PCR screening
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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