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Team:Paris/August 15
From 2008.igem.org
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=='''PCR amplification of flhDC and its promoter'''== | =='''PCR amplification of flhDC and its promoter'''== | ||
==='''List of PCRs'''=== | ==='''List of PCRs'''=== | ||
| + | {|PCR 136 | ||
| + | |PCR 137 | ||
| + | |PCR 138 | ||
| + | |PCR 136' | ||
| + | |PCR 137' | ||
| + | |PCR 138' | ||
| + | |sddvsv | ||
| + | |||
| + | |||
| + | |} | ||
| + | |||
==='''Protocol'''=== | ==='''Protocol'''=== | ||
==='''Results'''=== | ==='''Results'''=== | ||
Revision as of 14:31, 16 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells standard protocol. PCR amplification of flhDC and its promoterList of PCRs
ProtocolResultsDigestionsDigestionMeasurement of concentration of minipreps
DigestionLigation
Analysis of yesterday PCR screening
Starting the construction of the Promoter characterization plasmidDigestionMeasurement of concentration of minipreps
Digestion
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