Team:Paris/August 16

From 2008.igem.org

(Difference between revisions)

Revision as of 16:54, 16 August 2008

Analysis of the transformation we did yesterday

L143, L144, T1 and T2 showed no colonies. The positive control with pUC19 worked well.
We suppose that the ligation did not work. We will do it again today.

Ligation

Transformation

Transformation protocol

Name	Description	Antibio
Ligations
L150	D105(BV) - D146(BI) pLas - strongest rbs-TetR-GFP tripart	Amp
L151	D147(FI) - D125 (FV) strongest rbs-LasR activator with LVA - Double terminator	Kan
Controls
C1	D105	Amp
C2	D125	Kan
Positive Control	pUC19	Amp

@@ Line 35: / Line 35: @@
 |Positive Control
 |pUC19
-|
 |Amp
+|}