Team:Paris/August 20
From 2008.igem.org
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|14.5 | |14.5 | ||
|XbaI and PstI | |XbaI and PstI | ||
+ | |} | ||
+ | |||
+ | |||
+ | ==='''Gel Extraction'''=== | ||
+ | [[Image:KR000185b.jpg| thumb|Gel Extraction of D165]] | ||
+ | [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]] | ||
+ | |||
+ | |||
+ | {|border="1" style="text-align: center" | ||
+ | |'''Well''' | ||
+ | |'''Sample''' | ||
+ | |'''Expected size''' | ||
+ | |'''Measured size''' | ||
+ | |- | ||
+ | |1 | ||
+ | |1kb ladder | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |2 | ||
+ | |MP162.2 | ||
+ | |2855 | ||
+ | |style="background: #cbff7B"| 3000 | ||
+ | |- | ||
+ | |3 | ||
+ | |no sample | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |4 | ||
+ | |D165.2 | ||
+ | |2057 / 798 | ||
+ | |style="background: #cbff7B"| 2000 / 800 | ||
+ | |- | ||
+ | |5 | ||
+ | |no sample | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |6 | ||
+ | |100pb ladder | ||
+ | | | ||
+ | | | ||
|} | |} |
Revision as of 16:22, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
DigestionAim : Construction of "pFlgA-RBS-YFP-dbl ter" Measurement of concentration of minipreps
Digestion
Gel Extraction
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