Team:Paris/August 20
From 2008.igem.org
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(→Screening of the cloning of E0240 and FlhDC+promotor) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
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<br>We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading. | <br>We have to try again, but with a stronger dilution of the bacteria or with a smaller volume of spreading. | ||
*Resuspension of some bacteria from the glycerol stock into 1 mL of LB+antibiotic | *Resuspension of some bacteria from the glycerol stock into 1 mL of LB+antibiotic | ||
- | *Dilution 10 and dilution 100 | + | *Dilution 10 and dilution 100 |
*Spreading of each dilution on a LB plate containing the right antibiotic | *Spreading of each dilution on a LB plate containing the right antibiotic | ||
*Overnight incubation | *Overnight incubation |
Revision as of 17:25, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Construction of pFlgA - YFP tripartAim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
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