Team:Paris/August 20
From 2008.igem.org
(Difference between revisions)
(→Stock Glycerol of New Biobrick) |
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|MP163.1 | |MP163.1 | ||
|rowspan="2"| B0032 | |rowspan="2"| B0032 | ||
- | |rowspan="2"| RBS | + | |rowspan="2"| [[Image:Icon_rbs.png]]<br>RBS |
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|MP163.2 | |MP163.2 | ||
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|MP164.1 | |MP164.1 | ||
|rowspan="2"| E0422 | |rowspan="2"| E0422 | ||
- | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]RBS+ ECFP+ LVA+ term | + | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP+ LVA+ term |
|- | |- | ||
|MP164.2 | |MP164.2 | ||
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|MP165.1 | |MP165.1 | ||
|rowspan="2"| E0430 | |rowspan="2"| E0430 | ||
- | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]RBS+ YFP+ LVA- term | + | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP+ LVA- term |
|- | |- | ||
|MP165.2 | |MP165.2 | ||
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|MP166.1 | |MP166.1 | ||
|rowspan="2"| E0432 | |rowspan="2"| E0432 | ||
- | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]RBS+ YFP LVA+ term | + | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ YFP LVA+ term |
|- | |- | ||
|MP166.2 | |MP166.2 | ||
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|MP167.1 | |MP167.1 | ||
|rowspan="2"| E0420 | |rowspan="2"| E0420 | ||
- | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]RBS+ ECFP LVA- term | + | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ ECFP LVA- term |
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|MP167.2 | |MP167.2 | ||
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|MP168.1 | |MP168.1 | ||
|rowspan="2"| I732078 | |rowspan="2"| I732078 | ||
- | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]RBS+ mRFP LVA+ term | + | |rowspan="2"| [[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS+ mRFP LVA+ term |
|- | |- | ||
|MP168.2 | |MP168.2 | ||
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='''Construction of pFlgA - YFP tripart (+/- LVA)'''= | ='''Construction of pFlgA - YFP tripart (+/- LVA)'''= |
Revision as of 18:18, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New BiobricksConstruction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol [[Image:]]
Minipreps
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