Team:Paris/August 20
From 2008.igem.org
(Difference between revisions)
(→Digestion) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
||
Line 1: | Line 1: | ||
{{Paris/Calendar_Links|August 19|August 21}} | {{Paris/Calendar_Links|August 19|August 21}} | ||
- | =Screening of the cloning of E0240 and FlhDC+promotor= | + | ='''Screening of the cloning of E0240 and FlhDC+promotor'''= |
==Spreading the clones in order to obtain single colonies== | ==Spreading the clones in order to obtain single colonies== | ||
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
Line 34: | Line 34: | ||
*Spreading of 100 µL of each dilution on a LB plate containing the right antibiotic | *Spreading of 100 µL of each dilution on a LB plate containing the right antibiotic | ||
*Overnight incubation (37°C) | *Overnight incubation (37°C) | ||
- | |||
='''Miniprep and stock glycerol'''= | ='''Miniprep and stock glycerol'''= |
Revision as of 18:27, 20 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New BiobricksConstruction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol [[Image:]]
Minipreps
|