Team:Paris/August 20
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==Digestion== | ==Digestion== | ||
- | We had a problem with a gel extraction so we have to make again the digestions | + | We had a problem with a gel extraction so we have to make again the digestions from yesterday |
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+ | Other digestions made: |
Revision as of 14:50, 21 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New Biobricks
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol [[Image:]]
Minipreps
Promoter characterization plasmidsLigationOur ligations from yesterday didn't work. The positive control for transformation worked. DigestionWe had a problem with a gel extraction so we have to make again the digestions from yesterday
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