Team:Paris/August 20
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(Difference between revisions)
AnaJimenez (Talk | contribs) (→Sequencing) |
AnaJimenez (Talk | contribs) (→Digestion) |
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[[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | ||
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|'''Enzymes''' | |'''Enzymes''' | ||
|- | |- | ||
- | |MP3. | + | |D179 |
+ | |MP3.4 | ||
|B0015 (double terminator B0010-B0012) - FV | |B0015 (double terminator B0010-B0012) - FV | ||
|4 | |4 | ||
|EcoRI and XbaI | |EcoRI and XbaI | ||
|- | |- | ||
- | | | + | |D180 |
- | |promoter J23101 - BV | + | |MP101. |
+ | |promoter J23101- BV | ||
|1 | |1 | ||
|SpeI and PstI | |SpeI and PstI | ||
|- | |- | ||
+ | |D181 | ||
|MP104 | |MP104 | ||
|PTet (Tet promoter) - BV | |PTet (Tet promoter) - BV | ||
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|SpeI and PstI | |SpeI and PstI | ||
|- | |- | ||
+ | |D182 | ||
|MP114 | |MP114 | ||
|TetR - FI | |TetR - FI | ||
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|EcoRI and SpeI | |EcoRI and SpeI | ||
|- | |- | ||
+ | |D183 | ||
+ | |MP119 | ||
+ | |promoter pBad - BV | ||
+ | |1 | ||
+ | |SpeI and PstI | ||
+ | |- | ||
+ | |D184 | ||
|MP143 | |MP143 | ||
+ | |gfp generator - BI | ||
+ | |2 | ||
+ | |SpeI and PstI | ||
+ | |- | ||
+ | |D185 | ||
+ | |MP163 | ||
|gfp generator - BI | |gfp generator - BI | ||
|2 | |2 | ||
|SpeI and PstI | |SpeI and PstI | ||
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==Ligation== | ==Ligation== |
Revision as of 16:45, 21 August 2008
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The plates obtained from the speading of yesterday can't be used because there are not single colonies.
Miniprep and stock glycerolStock Glycerol of New BiobrickMiniprep of New Biobricks
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionMeasurement of concentration of minipreps
Digestion
Gel Extraction
Results of the transformation we did yesterdayNumber of colonies
PCR Screening==> Protocol
Minipreps
Promoter characterization plasmidsLigationOur ligations from yesterday didn't work. The positive control for transformation worked. DigestionWe had a problem with a gel extraction so we have to make again the digestions from yesterday
Ligation
Sequencing
Digestion
ScreeningGel 1
Gel 2
Conclusion => the sequence of pFlgB and pFlhB are not good we will tried to isolate pFlhB again. |