Revision as of 17:08, 25 August 2008

Construction of pFlgA - GFP Generator

Aim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0430/E0432)

Ligation name	Description	Antibio	Number Colonies observed	Fluorescence	Comments
Ligations
L158	D110(BV) - D131(BI) rbs-TetR - gfp tripart	Amp	-	-	to do again
L159	D125(FV) - D109(FI) rbs-LasI - double terminator	Kan	-	-	to do again
L162	D107(BV) - D163(BI) rbs-LasI - gfp generator	Amp	-	-	to do again
L163	D110(BV) - D163(BI) rbs-TetR - gfp generator	Amp	-	-	to do again
Controls
TL158	D110(BV)	Amp	-	-	to do again
TL159	D125(FV)	Kan	-	-	to do again
TL162	D107(BV)	Amp	-	-	to do again
TL163	D110(BV)	Amp	-	-	to do again
Positive Control	pUC19	Amp	358 (efficiency 3.5.10^7)	No	OK

=> We decided to do again the digestions of the ligation's reactions

@@ Line 24: / Line 24: @@
 ='''Construction for Synchronization'''=
-=='''Results of the transformation we did [[Team:Paris/August 19  |yesterday]]'''==
+=='''Results of the transformation we did [[Team:Paris/August 12  |yesterday]]'''==
 ==='''Number of colonies'''===