Team:Paris/August 24

From 2008.igem.org

(Difference between revisions)
(→Ligation)
(→Ligation)
 
Line 134: Line 134:
|'''Volume Insert (µL)'''
|'''Volume Insert (µL)'''
|-
|-
-
|L160
+
|L159
-
|D166
+
|D125
-
|3.63
+
|6
-
|D132
+
|D109
-
|2.03
+
|3.86
|-
|-
-
|L161
+
|L162
-
|D167
+
|D107
-
|4.00
+
|5
-
|D132
+
|D163
-
|2.01
+
|5.3
|-
|-
-
|Control L160
+
|L163
-
|D166
+
|D110
-
|3.63
+
|7.5
 +
|D163
 +
|5.32
 +
|-
 +
|Control L159
 +
|D125
 +
|6
 +
| -
 +
| -
 +
|-
 +
|Control L162
 +
|D107
 +
|5
| -
| -
| -
| -
|-
|-
-
|Control L161
+
|Control L163
-
|D167
+
|D110
-
|4.00
+
|7.5
| -
| -
| -
| -
|}
|}

Latest revision as of 12:58, 25 August 2008

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Construction for synchronization

Gel extraction

Protocol

Gel 1 (no image sorry, we forgot to save it!)

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample 1kb ladder MP105.1 no sample D107 no sample D109 no sample MP106 no sample D110 no sample 100pb ladder
Expected size (pb) - 2764 - 2750
14 		- 2056
708 		- 2782 - 2768
14 		- -
Measured size (pb) - 2800 - 2750 - 2000
700 		- 2800 - 2750 - -

Gel 2

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample no sample 1kb ladder MP118.1 no sample D125 no sample MP143 no sample D163 no sample 100pb ladder no sample
Expected size (pb) - - 3318 - 3303
15 		- 3322 - 2432
890 		- - -
Measured size (pb) - - 2900 - 3300 - 2900 - 2500
900 		- - -

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L159 D125 6 D109 3.86
L162 D107 5 D163 5.3
L163 D110 7.5 D163 5.32
Control L159 D125 6 - -
Control L162 D107 5 - -
Control L163 D110 7.5 - -
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