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Team:Paris/August 25
From 2008.igem.org
(Difference between revisions)
(→Cloning of EnvZ*) |
(→Cloning of EnvZ*) |
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| - | =Cloning of EnvZ*= | + | ='''Cloning of EnvZ*'''= |
[[Image:KR000225.jpg|thumb|]] | [[Image:KR000225.jpg|thumb|]] | ||
[[Image:KR000226.jpg|thumb|Gel after excision]] | [[Image:KR000226.jpg|thumb|Gel after excision]] | ||
| + | |||
| + | |||
| + | ='''Construction for Synchronization'''= | ||
| + | =='''Results of the transformation we did [[Team:Paris/August 23 |the day before yesterday]]'''== | ||
| + | |||
| + | ==='''Number of colonies'''=== | ||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |'''Ligation name''' | ||
| + | |'''Description''' | ||
| + | |'''Antibio''' | ||
| + | |'''Number Colonies observed''' | ||
| + | |'''Fluorescence''' | ||
| + | |'''Comments''' | ||
| + | |- | ||
| + | |colspan="6" |Ligations | ||
| + | |- | ||
| + | |L164 | ||
| + | |D168(FV) - D132(FI)<br>pFlgA - gfp generator | ||
| + | |Amp | ||
| + | | 125 | ||
| + | | No | ||
| + | | ok | ||
| + | |colspan="6" |Controls | ||
| + | |- | ||
| + | |TL164 | ||
| + | |D168(FV) | ||
| + | |Amp | ||
| + | | 8 | ||
| + | | No | ||
| + | | ok | ||
| + | |- | ||
| + | |Positive Control | ||
| + | |pUC19 | ||
| + | |Amp | ||
| + | |2000 (efficiency 2.10^8) | ||
| + | |No | ||
| + | |OK | ||
| + | |} | ||
| + | |||
| + | => Need to screen to know which clones we can use for the of '''pFlgA promotor caracteristion'''. | ||
Revision as of 17:21, 25 August 2008
Construction for SynchronizationTransformation of the ligations we did yesterday
Cloning of EnvZ* 
Construction for SynchronizationResults of the transformation we did the day before yesterdayNumber of colonies
=> Need to screen to know which clones we can use for the of pFlgA promotor caracteristion. |
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