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TUDelft/19 August 2008
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The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below: | The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below: | ||
| - | [[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]] | + | [[Image:gelTUDelft1.jpg|thumb|left|Gel of IDI and ISPA, click picture to enlarge]] [[Image:gelTUDelft2.jpg|thumb|right|Gel of ATOB, click picture to enlarge]] |
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==Live stabs== | ==Live stabs== | ||
Revision as of 09:05, 26 August 2008
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
19th August
Testing the Primers
The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:
Live stabs
All the live stabs cultures have grown in 5 ml LB + A. We've made glycerol stocks, plated them and grown them in 50 ml LB medium + A o/n again to perform midiprep tomorrow.
