Team:Paris/August 26
From 2008.igem.org
(Difference between revisions)
(→PCR) |
(→PCR) |
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* pFlgB (O102-F / O103-R) | * pFlgB (O102-F / O103-R) | ||
* pFlhB (O108-F / O109-R) | * pFlhB (O108-F / O109-R) | ||
- | --- | + | |
+ | ---- | ||
+ | |||
*Program: Gradient | *Program: Gradient | ||
Denaturation : 98°C-5'<br> | Denaturation : 98°C-5'<br> | ||
- | Cycling 1 (3X) : 98°C-10'' | + | Cycling 1 (3X) : 98°C-10'' |
- | Gradient 61°C +/-10°C - 30'' | + | Gradient 61°C +/-10°C - 30'' |
- | 72°C-30'' | + | 72°C-30'' |
- | Cycling 2 (25X) : 98°C-10'' | + | Cycling 2 (25X) : 98°C-10'' |
- | 72°C-30'' | + | 72°C-30'' |
- | Elongation : 72°C-5' | + | Elongation : 72°C-5' |
- | --- | + | |
+ | ---- | ||
+ | |||
Vf=20µL<br> | Vf=20µL<br> | ||
H20=13,4µL<br> | H20=13,4µL<br> |
Revision as of 10:21, 26 August 2008
PCR
Denaturation : 98°C-5' Gradient 61°C +/-10°C - 30 72°C-30 Cycling 2 (25X) : 98°C-10 72°C-30 Elongation : 72°C-5' Vf=20µL Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |