Team:Paris/August 26
From 2008.igem.org
(→Electrophoresis settings) |
(→Results of the electrphoresis) |
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*Ladder 1kb | *Ladder 1kb | ||
*4µL DNA + 2µL Loading Blue | *4µL DNA + 2µL Loading Blue | ||
- | ===Results of the | + | ===Results of the electrophoresis=== |
+ | {| style="text-align: center;" border="1" | ||
+ | |- | ||
+ | |'''Name''' | ||
+ | |'''Gene''' | ||
+ | |'''Well''' | ||
+ | |'''Expected size''' | ||
+ | |'''Measured size''' | ||
+ | |- | ||
+ | |PCR 147 | ||
+ | |EnvZ* | ||
+ | |2 | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |T 147 | ||
+ | |nothing | ||
+ | |3 | ||
+ | |nothing | ||
+ | | | ||
+ | |- | ||
+ | |PCR 148 | ||
+ | |OmpR* | ||
+ | |4 | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |T 148 | ||
+ | |nothing | ||
+ | |5 | ||
+ | |nothing | ||
+ | | | ||
+ | |} | ||
=PCR= | =PCR= |
Revision as of 13:29, 26 August 2008
Extraction of EnvZ* and OmpR* from E. coli genomeName of the PCR
ProtocolProtocol
PCR Programme
ResutsElectrophoresis settings
Results of the electrophoresis
PCRPCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3
Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation
|