Team:Paris/August 26
From 2008.igem.org
(→Extraction of EnvZ* and OmpR* from E. coli genome) |
(→Resuts) |
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==Resuts== | ==Resuts== | ||
+ | [[Image:KR000239.jpg|thumbs|Results of the cloning of EnvZ* and OmpR*]] | ||
===Electrophoresis settings=== | ===Electrophoresis settings=== | ||
*Gel 1% agar | *Gel 1% agar | ||
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|2 | |2 | ||
|1412 bp | |1412 bp | ||
- | | | + | |1400 bp |
|- | |- | ||
|T 147 | |T 147 | ||
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|3 | |3 | ||
|nothing | |nothing | ||
- | | | + | |nothing |
|- | |- | ||
|PCR 148 | |PCR 148 | ||
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|4 | |4 | ||
|779 bp | |779 bp | ||
- | | | + | |800 bp |
|- | |- | ||
|T 148 | |T 148 | ||
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|5 | |5 | ||
|nothing | |nothing | ||
- | | | + | |nothing |
|} | |} | ||
+ | Conclusion : All the PCR worked perfectly well ! | ||
==Cleaning of the PCR products== | ==Cleaning of the PCR products== |
Revision as of 16:56, 26 August 2008
Extraction of EnvZ* and OmpR* from E. coli genomeName of the PCR
ProtocolProtocol
PCR Programme
ResutsElectrophoresis settings
Results of the electrophoresis
Conclusion : All the PCR worked perfectly well ! Cleaning of the PCR productsDigestion of the PCR productsPCRPCR1
Program: Gradient Vf=20µL PCR2
Program: promoter Vf=50µL PCR3
Program: PCRFliA1 Program: PCRFliA2 Program: PCRFliA3
Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation
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