Team:Paris/August 29

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
(→Cloning of EnvZ*)
Line 13: Line 13:
|9 µg/mL
|9 µg/mL
|1,59
|1,59
 +
|-
|D116 (pSB1A2)
|D116 (pSB1A2)
|13 µg/mL
|13 µg/mL

Revision as of 13:02, 29 August 2008

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Cloning of EnvZ*

Concentration measurement

DNA concentration A260 / A2801
D159 (EnvZ*) 9 µg/mL 1,59
D116 (pSB1A2) 13 µg/mL 1,29

Ligation

control insert / vector ratio
3 / 1 4 / 1
D159 (EnvZ*) 0 8,7 µL 11,6 µL
D116 (pSB1A2) 2 µL 2 µL 2 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
H2O (qsp 20 µL) 15 µL 6,3 µL 3,4 µL
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