Team:Paris/August 29

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
(→Cloning of EnvZ*)
Line 7: Line 7:
{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
|
|
-
|DNA concentration
+
|'''DNA concentration'''
-
|A260 / A2801
+
|'''A260 / A2801'''
|-
|-
|D159 (EnvZ*)
|D159 (EnvZ*)

Revision as of 13:03, 29 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Cloning of EnvZ*

Concentration measurement

DNA concentration A260 / A2801
D159 (EnvZ*) 9 µg/mL 1,59
D116 (pSB1A2) 13 µg/mL 1,29

Ligation

control insert / vector ratio
3 / 1 4 / 1
D159 (EnvZ*) 0 8,7 µL 11,6 µL
D116 (pSB1A2) 2 µL 2 µL 2 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
H2O (qsp 20 µL) 15 µL 6,3 µL 3,4 µL
Retrieved from "http://2008.igem.org/Team:Paris/August_29"